Syntaxin 1 Antibody (4H256): sc-58297
- Syntaxin 1 Antibody (4H256) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- raised against synaptic vesicle-containing fractions that were immunoprecipitated from human brain homogenates using anti-human Syntaxin 1 monoclonal antibodies
- recommended for detection of Syntaxin 1 of mouse, rat, human, Xenopus , bovine, porcine and feline origin by WB, IP and IF; may cross-react with higher molecular weight proteins of unknown identity; non cross-reactive with a 35kDa protein in mouse liver extracts
- See Syntaxin 1 (HPC-1): sc-12736 for Syntaxin 1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Syntaxin 1 Antibody (4H256) for WB applications. These reagents are now offered in bundles with Syntaxin 1 Antibody (4H256) (see ordering information below).
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Syntaxin 1 Antibody (4H256) is a mouse monoclonal IgG1 antibody that detects Syntaxin 1 across multiple species including mouse, rat, human, Xenopus, bovine, porcine, and feline, and is suitable for western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Syntaxin 1 plays a crucial role in synaptic transmission, acting as a key component of the SNARE complex that facilitates the fusion of synaptic vesicles with the plasma membrane. Syntaxin 1 is primarily located in presynaptic terminals of neurons, where anti-Syntaxin 1 antibody (4H256) can detect essential neurotransmitter release functions. Syntaxin 1 (4H256) monoclonal antibody enables research into synaptic plasticity and brain function, as disruptions in Syntaxin 1 activity can lead to various neurological disorders. Syntaxin 1 interacts with VAMP and SNAP-25, forming a stable SNARE complex critical for vesicle docking and fusion. This intricate interplay underscores Syntaxin 1′s importance in maintaining effective synaptic transmission and highlights potential therapeutic interventions in synaptic dysfunction. Syntaxin 1 (4H256) antibody serves as an invaluable tool for studying vesicular transport and synaptic function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Syntaxin 1 Antibody (4H256) References:
- A syntaxin 1, Galpha(o), and N-type calcium channel complex at a presynaptic nerve terminal: analysis by quantitative immunocolocalization. | Li, Q., et al. 2004. J Neurosci. 24: 4070-81. PMID: 15102922
- De novo protein synthesis of syntaxin-1 and dynamin-1 in long-term memory formation requires CREB1 gene transcription in Lymnaea stagnalis. | Guo, CH., et al. 2010. Behav Genet. 40: 680-93. PMID: 20563839
- PTPRT regulates the interaction of Syntaxin-binding protein 1 with Syntaxin 1 through dephosphorylation of specific tyrosine residue. | Lim, SH., et al. 2013. Biochem Biophys Res Commun. 439: 40-6. PMID: 23962429
- Blockade of the SNARE protein syntaxin 1 inhibits glioblastoma tumor growth. | Ulloa, F., et al. 2015. PLoS One. 10: e0119707. PMID: 25803850
- SNARE Protein Syntaxin-1 Colocalizes Closely with NMDA Receptor Subunit NR2B in Postsynaptic Spines in the Hippocampus. | Hussain, S., et al. 2016. Front Mol Neurosci. 9: 10. PMID: 26903802
- Distinct Functions of Syntaxin-1 in Neuronal Maintenance, Synaptic Vesicle Docking, and Fusion in Mouse Neurons. | Vardar, G., et al. 2016. J Neurosci. 36: 7911-24. PMID: 27466336
- Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters. | Toft-Bertelsen, TL., et al. 2016. Mol Biol Cell. 27: 3329-3341. PMID: 27605709
- rbSec1A and B colocalize with syntaxin 1 and SNAP-25 throughout the axon, but are not in a stable complex with syntaxin. | Garcia, EP., et al. 1995. J Cell Biol. 129: 105-20. PMID: 7698978
- The t-SNAREs syntaxin 1 and SNAP-25 are present on organelles that participate in synaptic vesicle recycling. | Walch-Solimena, C., et al. 1995. J Cell Biol. 128: 637-45. PMID: 7860636
- Expression and functional role of syntaxin 1/HPC-1 in pancreatic beta cells. Syntaxin 1A, but not 1B, plays a negative role in regulatory insulin release pathway. | Nagamatsu, S., et al. 1996. J Biol Chem. 271: 1160-5. PMID: 8557645
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Syntaxin 1 Antibody (4H256) | sc-58297 | 200 µg/ml | $322.00 | |||
Syntaxin 1 Antibody (4H256): m-IgG Fc BP-HRP Bundle | sc-537147 | 200 µg Ab; 10 µg BP | $361.00 | |||
Syntaxin 1 Antibody (4H256): m-IgGκ BP-HRP Bundle | sc-534342 | 200 µg Ab; 40 µg BP | $361.00 | |||
Syntaxin 1 Antibody (4H256): m-IgG1 BP-HRP Bundle | sc-545036 | 200 µg Ab; 20 µg BP | $361.00 |