Synaptojanin 2 Antibody (D-11): sc-390247
- Synaptojanin 2 Antibody (D-11) is a mouse monoclonal IgG2a κ Synaptojanin 2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1109-1135 at the C-terminus of Synaptojanin 2 of mouse origin
- Synaptojanin 2 Antibody (D-11) is recommended for detection of all Synaptojanin 2 isoforms (α-Ω) of mouse origin and Synaptojanin 2 of rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and bovine and porcine
- Anti-Synaptojanin 2 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Synaptojanin 2 Antibody (D-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with Synaptojanin 2 Antibody (D-11) (see ordering information below).
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Synaptojanin 2 Antibody (D-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects Synaptojanin 2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Synaptojanin 2 antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Synaptojanin 2 plays a crucial role in synaptic vesicle endocytosis, essential for neurotransmitter release and synaptic plasticity - processes vital for proper neuronal communication and function. Located primarily in brain tissue, Synaptojanin 2 regulates actin cytoskeleton, maintaining cell shape and facilitating intracellular transport. Synaptojanin 2 features a unique structure with an N-terminal SAC1-like sequence, central 5-phosphate domain, and distinct C-terminal sequence, enabling interactions with various phosphoinositides and participation in signaling pathways. Multiple isoforms of Synaptojanin 2 exist due to alternative splicing, with eight splice variants identified, including a brain-specific isoform, highlighting diverse functional roles in different tissues. Synaptojanin 2′s ability to modulate synaptic vesicle dynamics and cytoskeletal organization underscores its importance in both normal physiology and potential pathophysiological conditions, making Synaptojanin 2 (D-11) antibody a valuable research tool for studying synaptic function and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Synaptojanin 2 Antibody (D-11) References:
- Regulation of phosphatidylinositol 4,5-bisphosphate levels and its roles in cytoskeletal re-organization and malignant transformation. | Takenawa, T., et al. 1999. Chem Phys Lipids. 98: 13-22. PMID: 10358924
- Direct interaction of the 170 kDa isoform of synaptojanin 1 with clathrin and with the clathrin adaptor AP-2. | Haffner, C., et al. 2000. Curr Biol. 10: 471-4. PMID: 10801423
- Regulation of second messengers by the inositol polyphosphate 5-phosphatases. | Mitchell, CA., et al. 1996. Biochem Soc Trans. 24: 994-1000. PMID: 8968499
- Synaptojanin 2, a novel synaptojanin isoform with a distinct targeting domain and expression pattern. | Nemoto, Y., et al. 1997. J Biol Chem. 272: 30817-21. PMID: 9388224
- Developmentally regulated alternative splicing in a novel synaptojanin. | Khvotchev, M. and Südhof, TC. 1998. J Biol Chem. 273: 2306-11. PMID: 9442075
- Phosphatidylinositol signalling reactions. | Zhang, X. and Majerus, PW. 1998. Semin Cell Dev Biol. 9: 153-60. PMID: 9599410
- Molecular cloning of multiple isoforms of synaptojanin 2 and assignment of the gene to mouse chromosome 17A2-3.1. | Seet, LF., et al. 1998. Biochem Biophys Res Commun. 247: 116-22. PMID: 9636665
- The diversity and possible functions of the inositol polyphosphate 5-phosphatases. | Erneux, C., et al. 1998. Biochim Biophys Acta. 1436: 185-99. PMID: 9838104
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synaptojanin 2 Antibody (D-11) | sc-390247 | 200 µg/ml | $322.00 | |||
Synaptojanin 2 Antibody (D-11): m-IgG Fc BP-HRP Bundle | sc-526137 | 200 µg Ab; 10 µg BP | $361.00 | |||
Synaptojanin 2 Antibody (D-11) AC | sc-390247 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Synaptojanin 2 Antibody (D-11) HRP | sc-390247 HRP | 200 µg/ml | $322.00 | |||
Synaptojanin 2 Antibody (D-11) FITC | sc-390247 FITC | 200 µg/ml | $336.00 | |||
Synaptojanin 2 Antibody (D-11) PE | sc-390247 PE | 200 µg/ml | $349.00 | |||
Synaptojanin 2 Antibody (D-11) Alexa Fluor® 488 | sc-390247 AF488 | 200 µg/ml | $364.00 | |||
Synaptojanin 2 Antibody (D-11) Alexa Fluor® 546 | sc-390247 AF546 | 200 µg/ml | $364.00 | |||
Synaptojanin 2 Antibody (D-11) Alexa Fluor® 594 | sc-390247 AF594 | 200 µg/ml | $364.00 | |||
Synaptojanin 2 Antibody (D-11) Alexa Fluor® 647 | sc-390247 AF647 | 200 µg/ml | $364.00 | |||
Synaptojanin 2 Antibody (D-11) Alexa Fluor® 680 | sc-390247 AF680 | 200 µg/ml | $364.00 | |||
Synaptojanin 2 Antibody (D-11) Alexa Fluor® 790 | sc-390247 AF790 | 200 µg/ml | $364.00 | |||
Synaptojanin 2 (D-11) Neutralizing Peptide | sc-390247 P | 100 µg/0.5 ml | $69.00 |