SPT6 Double Nickase Plasmid (h): sc-406219-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPT6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SPT6 Double Nickase Plasmid (h) and SPT6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SUPT6H. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SPT6 Antibody (E-4): sc-393920
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPT6 Double Nickase Plasmid (h) | sc-406219-NIC | 20 µg | $410.00 |
SUPT6H encodes SPT6, a conserved histone chaperone and transcription elongation factor that associates with RNA polymerase II to coordinate nucleosome reassembly and preserve chromatin integrity during transcription. By interacting with factors such as the PAF1 complex and regulating histone modification patterns, SPT6 links transcriptional elongation to co-transcriptional RNA processing and epigenetic maintenance. This activity supports global gene expression programs that govern cell identity, proliferation, and stress responses. Dysregulation of SPT6-dependent chromatin control has been implicated in aberrant transcriptional states observed in cancer and other disorders of transcription and genome regulation.
SPT6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SUPT6H locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SUPT6H. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SUPT6H function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SUPT6H-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.