SPF30 Antibody (E-5): sc-398258

SPF30 Antibody (E-5) is a mouse monoclonal IgG2a antibody that detects SPF30 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPF30, also known as survival motor neuron domain containing 1 (SMNDC1) or SMN-related protein (SMNR), is a crucial splicing factor that plays a vital role in the assembly of the spliceosome, a complex responsible for the removal of introns from pre-mRNA. SPF30 is predominantly expressed in skeletal muscle, pancreas, and heart tissues, where SPF30 localizes to Cajal bodies and nuclear speckles, sites essential for RNA processing. The structure of SPF30 includes a Tudor domain that shares significant similarity with the Survival Motor Neuron (SMN) protein, highlighting SPF30′s importance in the recruitment of U snRNPs, which are integral components of the spliceosome. Loss of SPF30 function leads to a halt in spliceosome assembly at the prespliceosome stage, underscoring SPF30′s critical role in mediating the incorporation of the U4/U5/U6 tri-snRNP complex. Overexpression of SPF30 has been linked to apoptosis, indicating that SPF30 regulation is crucial for cellular health and function. With multifaceted roles in RNA splicing and cellular processes, anti-SPF30 antibody (E-5) serves as an invaluable tool for researchers investigating the mechanisms of gene expression and the implications of splicing factors in various diseases.

The preferred SPF30 antibody is SPF30 Antibody (G-8): sc-376887.