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RUNX1 Antibody (DW71) - Western Blotting - Image 138603
Anticorpo RUNX1 (DW71): sc-101146. Análise de Western blot da expressão de RUNX1 em extractos nucleares de SH-SY5Y (A), HeLa (B), HL-60 (C), THP-1 (D) e U-937 (E).
Anticorpo RUNX1 (DW71): sc-101146. Análise de Western blot da expressão de RUNX1 em extractos nucleares de SH-SY5Y (A), HeLa (B), HL-60 (C), THP-1 (D) e U-937 (E).

RUNX1 Anticorpo (DW71): sc-101146

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Fichas de dados
  • RUNX1 Anticorpo DW71é um anticorpo monoclonal produzido em camundongo IgG2b κ, citado em 15 publicações, fornecido em 100 µg/ml
  • Produzido contra recombinante RUNX1 de human origem
  • recomendado para a detecção de a broad range of RUNX1 isoforms de mouse, rat e human origem em
  • See RUNX1 (A-2): sc-365644 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de RUNX1 (DW71): sc-101146.
  • Atualmente, ainda não concluímos a identificação do(s) reagente(s) de deteção secundário(s) preferido(s) para RUNX1 Anticorpo (DW71). Este trabalho está em curso.

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Produtos auxiliares
Descricao
Identificacao do gene
Ficha de dados e Protocolos
Informações de investigação

VEJA TAMBÉM

Adicionais RUNX Anticorpos incluindo RUNX1, RUNX2 e RUNX3

O Anticorpo RUNX1 (DW71) é um anticorpo monoclonal RUNX1 IgG2b κ de camundongo que detecta uma ampla gama de isoformas RUNX1 de origem camundongo, rata e humana por WB, IP, IF, IHC(P) e ELISA. O anticorpo RUNX1 (DW71) está disponível na forma de anticorpo anti-RUNX1 não conjugado. A família de fatores de transcrição relacionados a Runt (RUNX) de mamíferos compreende três membros, RUNX1 (também designado AML-1, PEBP2αB, CBFA2), RUNX2 (também designado AML-3, PEBP2αA, CBFA1, Osf2) e RUNX3 (também designado AML-2, PEBPαC, CBFA3). Os membros da família genética RUNX são proteínas de ligação protéica ao DNA que regulam a expressão de genes envolvidos na diferenciação celular e na progressão do ciclo celular. A RUNX1 está envolvida na hematopoiese e frequentemente se transloca para a leucemia humana por meio de translocações cromossômicas que fundem o domínio de ligação ao DNA da RUNX1 a outros fatores de transcrição e moléculas de corepressão. Além de sua função na leucemogênese, o RUNX1 também está envolvido na diversificação dos neurônios sensoriais. Especificamente, o RUNX1 promove o crescimento axonal, é expresso seletivamente em neurônios sensoriais TrkA+ derivados da crista neural e medeia a transativação de TrkA em células migratórias da crista neural. As emendas alternativas dão origem a várias isoformas de RUNX1.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

RUNX1 Anticorpo (DW71)

sc-101146
100 µg/ml
$333.00

Hello, is this antibody recognize the N terminal or C terminal of RUNX1?

Asked by: YIZHEN
Thank you for your question. This RUNX1 antibody was raised against recombinant protein mapping within amino acids 210-310 of RUNX1 of human origin. The exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2019-10-10

Does the antibody RUNX1 Antibody (DW71): sc-101146 raise against which part of the Runx1?

Asked by: sunny Zhang
Thank you for your question. Please contact our Technical Service Department and we can assist you by providing an amino acid range within where the epitope is mapping for this antibody.
Answered by: Technical Support
Date published: 2019-01-25

I was wondering which method for antigen retrieval (IHCp) is proposed for this antibody? I tried heated citric acid buffer, varied dilutions of the antibody from 1:50-500 and did not get any signal. Thanks for your help.

Asked by: Friederike Philipp
Thank you for your question. We recommend using one of three methods for antigen retrieval: Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. Pepsin: Incubate sections for 10–20 minutes in 0.1% Pepsin (sc-476554) in 0.01 N HCl at room temperature. Wash slides several times in deionized H2O. Aspirate excess liquid from slides. Saponin: Incubate sections for 30 minutes in 0.05% Saponin (sc-280079) in deionized H2O at room temperature. Wash at least three times in PBS. Aspirate excess liquid from slides. You can find our complete recommended protocol by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunofluorescence-cell-staining If you are still having trouble with this antibody, please contact our Technical Service by phone or email or by Live Chat and if we cannot resolve the issue, we will provide an appropriate replacement free of charge.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 7
Date published: 2017-02-15
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Rated 5 out of 5 by from Good for IHC staining on fixed mouse muscleThe antibody works well when I do immunostaining on sections cut from fixed mouse muscles.
Date published: 2023-05-24
Rated 5 out of 5 by from Good specificityFairly clean Western Blot banding and nuclear localization in IF and IHC.
Date published: 2017-01-18
Rated 5 out of 5 by from good functionIt worked very well and the background was clean. Happy to use it.
Date published: 2016-11-24
Rated 5 out of 5 by from Used successfully in WBUsed successfully in WB. Published - See PubMed ID 23442798.
Date published: 2015-09-29
Rated 5 out of 5 by from Strong band in WB in human HAFTL preStrong band in WB in human HAFTL pre-B cells and RAW264.7 cells. -SCBT Publication Review
Date published: 2015-04-23
Rated 5 out of 5 by from Good publishable data in WB in human AsPc1Good publishable data in WB in human AsPc1, HeLa, RKO, HEK293, and MSTO-211H cell lines. -SCBT Publication Review
Date published: 2015-04-23
Rated 5 out of 5 by from Robust signal in WB in SHRobust signal in WB in SH-SY5Y, HeLa, HL-60, THP-1 and U-937 nuclear extracts. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Worked well in ChiP and IP in mouse and humanWorked well in ChiP and IP in mouse and human 2D2 mouse T cell, Resting (Pan) CD4 T cells, Human CD4 T cell. -SCBT Publication Review
Date published: 2015-02-12
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RUNX1 Anticorpo (DW71) is rated 5.0 out of 5 by 10.
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