Rim1/2 Antibody (B-4): sc-515918

Rim1/2 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rim1 and Rim2 proteins of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Rim1/2 (B-4) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rim proteins play a crucial role in synaptic transmission, particularly in regulating synaptic vesicle fusion, which is essential for neurotransmitter release. Rim1 and Rim2, produced by alternative splicing, contain distinct structural domains that facilitate interaction with Rab3, a key player in calcium-regulated exocytosis. The amino-terminal zinc-finger motif and carboxy-terminal PDZ and C2 domains of Rim proteins form a GTP-dependent complex with Rab3 at the synapse, enhancing recruitment of synaptic vesicles to the plasma membrane. This interaction controls neurotransmitter release, which underpins synaptic plasticity and communication between neurons. Rim proteins are also localized to the plasma membrane of INS-1E cells and pancreatic beta-cells, where these proteins regulate insulin secretion, highlighting their importance in both neural and endocrine signaling pathways.

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Rim1/2 Antibody (B-4) References:

1. Disruption of Rab3-calmodulin interaction, but not other effector interactions, prevents Rab3 inhibition of exocytosis.  |  Coppola, T., et al. 1999. EMBO J. 18: 5885-91. PMID: 10545100
2. The RIM/NIM family of neuronal C2 domain proteins. Interactions with Rab3 and a new class of Src homology 3 domain proteins.  |  Wang, Y., et al. 2000. J Biol Chem. 275: 20033-44. PMID: 10748113
3. The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis.  |  Iezzi, M., et al. 2000. FEBS Lett. 474: 66-70. PMID: 10828453
4. cAMP-GEFII is a direct target of cAMP in regulated exocytosis.  |  Ozaki, N., et al. 2000. Nat Cell Biol. 2: 805-11. PMID: 11056535
5. A direct inhibitory role for the Rab3-specific effector, Noc2, in Ca2+-regulated exocytosis in neuroendocrine cells.  |  Haynes, LP., et al. 2001. J Biol Chem. 276: 9726-32. PMID: 11134008
6. RIM1 and RIM2 redundantly determine Ca2+ channel density and readily releasable pool size at a large hindbrain synapse.  |  Han, Y., et al. 2015. J Neurophysiol. 113: 255-63. PMID: 25343783
7. RIM1/2-Mediated Facilitation of Cav1.4 Channel Opening Is Required for Ca2+-Stimulated Release in Mouse Rod Photoreceptors.  |  Grabner, CP., et al. 2015. J Neurosci. 35: 13133-47. PMID: 26400943
8. RIM1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation.  |  Assali, A., et al. 2017. Sci Rep. 7: 3236. PMID: 28607399
9. An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury.  |  Hilton, BJ., et al. 2022. Neuron. 110: 51-69.e7. PMID: 34706221
10. Structural and functional reorganization of inhibitory synapses by activity-dependent cleavage of neuroligin-2.  |  Xu, N., et al. 2024. Proc Natl Acad Sci U S A. 121: e2314541121. PMID: 38657049
11. Functional specificity of liquid-liquid phase separation at the synapse.  |  Guzikowski, NJ. and Kavalali, ET. 2024. Nat Commun. 15: 10103. PMID: 39572583
12. Rim is a putative Rab3 effector in regulating synaptic-vesicle fusion.  |  Wang, Y., et al. 1997. Nature. 388: 593-8. PMID: 9252191

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rim1/2 Antibody (B-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Rim1/2 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rim1/2 Antibody (B-4) Specifications

Alternate Product Name: Rim1/2

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 27-54

Epitope Target: Rim1

Epitope Species: human

Product Reactivity/Detection: Rim1 and Rim2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Rim1/2 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin