Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PSM CRISPR/Cas9 KO Plasmid (h): sc-401947
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSM CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PSM genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PSM Antibody (F-2): sc-514444
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSM CRISPR/Cas9 KO Plasmid (h) | sc-401947 | 20 µg | $397.00 |
Overview
FOLH1 encodes prostate-specific membrane antigen (PSM), a type II transmembrane metallopeptidase with folate hydrolase and NAALADase activity that modulates extracellular glutamate availability and folate metabolism. PSM participates in peptidase-dependent processing of polyglutamated folates and neuropeptides, influencing amino acid signaling and nutrient handling at the cell surface. In humans, FOLH1 expression is widely used as a molecular marker in prostate biology and is also detected in tumor-associated neovasculature, making it relevant to studies of cancer cell signaling, microenvironmental interactions, and lineage-specific regulation. Altered PSM activity and expression have been linked to disease-associated changes in metabolism and receptor-mediated trafficking, supporting mechanistic investigations in oncology and neurobiology models.
PSM CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FOLH1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FOLH1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FOLH1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PSM protein expression.
This CRISPR knockout system enables efficient generation of FOLH1-deficient cell models for investigation of PSM signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FOLH1 exon(s) critical for PSM function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FOLH1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PSM CRISPR/Cas9 KO Plasmid (h) and PSM CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FOLH1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PSM HDR Plasmid (h) and PSM HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FOLH1 homology arms to support homology-directed repair at defined FOLH1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.