Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
POMT2 CRISPR Activation Plasmid (h): sc-409912-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- POMT2 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- POMT2 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by POMT2 CRISPR Activation Plasmid (h) and POMT2 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the POMT2 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: POMT2 Antibody (G-3): sc-393487
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POMT2 CRISPR Activation Plasmid (h) | sc-409912-ACT | 20 µg | $397.00 |
POMT2 (protein O-mannosyltransferase 2) is an endoplasmic reticulum–resident glycosyltransferase that, together with POMT1, catalyzes the initial step of protein O-mannosylation. This modification is essential for the maturation and function of multiple membrane and secreted glycoproteins, most notably α-dystroglycan, thereby influencing extracellular matrix interactions, basement membrane integrity, and muscle and neuronal development. POMT2 activity integrates with glycosylation and secretory pathway quality control processes that shape cell adhesion, migration, and tissue architecture. Disrupted POMT2 function is associated with dystroglycanopathies and related neuromuscular and neurodevelopmental phenotypes, making it a relevant target for mechanistic studies of glycoprotein processing and dystroglycan signaling.
POMT2 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous POMT2 expression without altering the underlying DNA sequence.
POMT2 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the POMT2 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the POMT2 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous POMT2 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native POMT2 locus and enabling the study of POMT2-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of POMT2 pathway restoration in tumor cells with silenced or reduced POMT2 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.