PMS2 CRISPR/Cas9 KO Plasmid (m): sc-422324
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PMS2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PMS2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PMS2 Antibody (B-3): sc-25315
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PMS2 CRISPR/Cas9 KO Plasmid (m) | sc-422324 | 20 µg | $397.00 |
Overview
Mouse Pms2 encodes the PMS2 protein, a core component of the MutLα mismatch repair complex with MLH1 that corrects base–base mismatches and insertion–deletion loops arising during DNA replication. PMS2 contributes endonuclease activity that coordinates excision and resynthesis steps, supporting genome stability and proper DNA damage signaling. Through its role in post-replicative repair and maintenance of microsatellite integrity, altered PMS2 function is linked to elevated mutation rates and dysregulated responses to replication stress. In research settings, Pms2 perturbation is used to model mismatch repair deficiency and to study mutation accumulation, DNA repair pathway crosstalk, and checkpoint responses.
PMS2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pms2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pms2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pms2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PMS2 protein expression.
This CRISPR knockout system enables efficient generation of Pms2-deficient cell models for investigation of PMS2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pms2 exon(s) critical for PMS2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pms2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PMS2 CRISPR/Cas9 KO Plasmid (m) and PMS2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pms2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PMS2 HDR Plasmid (m) and PMS2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pms2 homology arms to support homology-directed repair at defined Pms2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.