Date published: 2025-9-8

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PGC-1α Anticorpo (D-5): sc-518025

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Fichas de dados
  • PGC1a Anticorpo D-5é um anticorpo monoclonal produzido em camundongo IgG2a κ PGC1a anticorpo, citado em 125 publicações, fornecido em 200 µg/ml
  • raised against amino acids 1-300 mapping near the N-terminus of of human origin
  • PGC1a Anticorpo (D-5) é recomendado para a detecção de PGC-1α de mouse, rat e human origem em WB, IP, IF, IHC(P) e ELISA
  • Anticorpo anti-PGC1a O anticorpo anti-PGC1a (D-5) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de PGC1a (D-5): sc-518025.
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP e m-IgGκ BP-HRP são os reagentes de deteção secundários preferidos para PGC1a Anticorpo (D-5) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com PGC1a Anticorpo (D-5) (ver informações para encomenda abaixo).

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VEJA TAMBÉM

O Anticorpo PGC-1α (D-5) é um anticorpo monoclonal de cadeia leve IgG2a kappa de camundongo concebido para reconhecer proteínas PGC-1α de origem de camundongo, rato e humana, visando os aminoácidos 1-300 perto do N-terminal. O PGC-1α, ou coactivador 1-alfa do recetor gama ativado por proliferador de peroxissoma, é um coactivador essencial da transcrição nuclear que orquestra a regulação dos genes envolvidos no metabolismo energético, na biogénese mitocondrial e na termogénese adaptativa. Localizado predominantemente no núcleo, a presença nuclear do PGC-1α é crucial, uma vez que o PGC-1α permite a interação direta com vários factores de transcrição, facilitando o controlo preciso da expressão genética em resposta às necessidades energéticas celulares e ao stress metabólico. Esta localização estratégica permite à PGC-1α coordenar eficazmente as respostas celulares que mantêm a homeostase energética e apoiam a função mitocondrial. Além disso, a PGC-1α sofre extensas modificações pós-traducionais, incluindo fosforilação, acetilação, metilação e ubiquitinação, que são essenciais para modular a atividade, a estabilidade e as interações com outras proteínas. Estas modificações permitem à PGC-1α responder dinamicamente a diversos sinais celulares e estímulos ambientais, assegurando uma regulação flexível e eficiente das vias metabólicas. O anticorpo anti-PGC-1α (D-5) é uma ferramenta indispensável para os investigadores, oferecendo um desempenho robusto em aplicações como western blotting (WB), imunoprecipitação (IP), imunofluorescência (IF), imunohistoquímica com secções incluídas em parafina (IHCP) e ensaio de imunoabsorção enzimática (ELISA). Ao facilitar investigações detalhadas sobre os papéis multifacetados do PGC-1α, o anticorpo anti-PGC-1α (D-5) apoia estudos avançados sobre a regulação metabólica, a dinâmica mitocondrial e os mecanismos moleculares subjacentes a vários processos fisiológicos e patológicos, contribuindo assim significativamente para a compreensão da gestão da energia celular e da integridade genómica.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

PGC1a Anticorpo (D-5)

sc-518025
200 µg/ml
$316.00

Pacote do PGC1a (D-5): m-IgG Fc BP-HRP

sc-531597
200 µg Ab; 10 µg BP
$354.00

Pacote do PGC1a (D-5): m-IgGκ BP-HRP

sc-525331
200 µg Ab, 40 µg BP
$354.00

Pacote do PGC1a (D-5): m-IgG2a BP-HRP

sc-547825
200 µg Ab; 10 µg BP
$354.00

PGC1a Anticorpo (D-5) AC

sc-518025 AC
500 µg/ml, 25% agarose
$416.00

PGC1a Anticorpo (D-5) HRP

sc-518025 HRP
200 µg/ml
$316.00

PGC1a Anticorpo (D-5) FITC

sc-518025 FITC
200 µg/ml
$330.00

PGC1a Anticorpo (D-5) PE

sc-518025 PE
200 µg/ml
$343.00

PGC1a Anticorpo (D-5) Alexa Fluor® 488

sc-518025 AF488
200 µg/ml
$357.00

PGC1a Anticorpo (D-5) Alexa Fluor® 546

sc-518025 AF546
200 µg/ml
$357.00

PGC1a Anticorpo (D-5) Alexa Fluor® 594

sc-518025 AF594
200 µg/ml
$357.00

PGC1a Anticorpo (D-5) Alexa Fluor® 647

sc-518025 AF647
200 µg/ml
$357.00

PGC1a Anticorpo (D-5) Alexa Fluor® 680

sc-518025 AF680
200 µg/ml
$357.00

PGC1a Anticorpo (D-5) Alexa Fluor® 790

sc-518025 AF790
200 µg/ml
$357.00

Is N-terminal PGC1a 37kd equivalent to the full-length PGC1a 110kd?

Asked by: gaf56876
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Sen Li
Date published: 2024-04-01

Has this antibody been validated for the application in Flow cytometry analysis ? (especially for mouse samples)

Asked by: 210715
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I am also interested in PGC1a sc-518028 antibody for use in Chromatin Immunoprecipitation and Immunofluorescence staining. Can you confirm that this works?

Asked by: Jeff20
Thank you for your question. We have not yet tested this antibody, nor are we aware of any publications citing use of this antibody for Chromatin Immunoprecipitation (ChIP). This antibody is recommended for immunofluorescent staining (IF) and we have published data available on our website now. There are also multiple product citations using this antibody for IF.
Answered by: Technical Support
Date published: 2019-02-10

I have two questions for the antibody.1: What is the exact molecule weight for WB, 130 or 100KD? 2. Can the antibody be used for flow analysis if I add Alexa Fluor® 488 antibody after incubation?

Asked by: Shaowei
Thank you for your question. The predicted molecular weight for full length PGC-1α1 is ~90 kDa, with the expected observed molecular weight ~115 kDa. This antibody has an N-terminal epitope and we have data detecting a smaller molecular weight isoform, NT-PGC-1α (NT(erminal)-PGC-1α, ~ 37 kDa. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-02-06

I'm interested in the new PGC-1α Antibody (D-5): sc-518025 for WB and IP in mouse liver lysates. However, in the datasheet I don't see a picture showing an endogenous full lenght PGC1a expression or a sucssefull IP, do you have such data? Thanks,

Asked by: Asael
Thanks for your question. The antibody is recommended for detection of PGC-1α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA and has been established for these applications. There are two publications that have successfully used PGC-1α Antibody (D-5): sc-518025 in western blot: PMID 30246714 and PMID. So far we have not received any customer feedback for IP with this antibody. 30515824. Please contact technical service for support or to arrange for a free sample of this antibody to see if it is suitable for your application.
Answered by: SCBT Heidelberg Support
Date published: 2019-02-10

你好,请问这个抗体可以申请免费的试用装吗?

Asked by: Alisen
感谢您的提问。关于试用装的申请,欢迎通过:电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For sample request, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-05-02
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Rated 5 out of 5 by from 抗体质量好我买过很多支这个产家的抗体,质量很好,效果稳定,特异性强,趋势明显,很多在其他产家找不到的抗体都能在这里找到,性价比高
Date published: 2024-01-18
Rated 5 out of 5 by from Well published monoclonal antibodyThis antibody detects the NT-PGC-1α form consistently in Western Blot and is highly published. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from GoodTested in mouse kidney extracts for wb and obtained good results
Date published: 2020-12-03
Rated 2 out of 5 by from debolequesto anticorpo dà un segnale specifico, ma debole
Date published: 2020-10-16
Rated 4 out of 5 by from Worked fine.We used this antibody for WB on lysate from mouse eye with the dilution 1:1000, and it worked fine.
Date published: 2020-10-07
Rated 4 out of 5 by from Worked okay for western blotThis antibody worked okay for western blot with 1:160 dilution. (100 ug protein, rat liver, using the recommended Santa Cruz secondary antibody). There was a faint signal (but detectable) and some background though.
Date published: 2019-07-26
Rated 1 out of 5 by from Not workingNot working Don't waste your time. I bought this instead of discontined SC-13067.
Date published: 2019-06-20
Rated 1 out of 5 by from Doesn't work don't waste time!Dear All, we have tested PGC1-alfa AB from sc and none of them work so far, don't waste time!
Date published: 2018-08-24
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PGC-1α Anticorpo (D-5) is rated 3.2 out of 5 by 9.
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