Pepsin A Antibody (A-10): sc-271798

Pepsin A Antibody (A-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects Pepsin A protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Pepsin A antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pepsin A plays a crucial role in the digestive system as one of the primary proteolytic enzymes secreted by the gastric mucosa, where Pepsin A is responsible for breaking down proteins into smaller peptides, facilitating nutrient absorption. Pepsin A is synthesized as an inactive precursor, pepsinogen, which is activated by the acidic environment of the stomach, specifically at a pH of 1-3, created by hydrochloric acid. This activation process is vital, as Pepsin A is only active in the stomach, preventing protein digestion in other parts of the body. Pepsin A exhibits broad specificity, effectively cleaving peptide bonds involving aromatic amino acids, with a preference for phenylalanine and leucine, making Pepsin A essential for proper digestion of dietary proteins. Additionally, Pepsin A is classified as a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase found in vertebrate gastric juice, highlighting Pepsin A′s importance in digestive physiology.

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Pepsin A Antibody (A-10) References:

1. The role of the flap residue, threonine 77, in the activation and catalytic activity of pepsin A.  |  Okoniewska, M., et al. 1999. Protein Eng. 12: 55-61. PMID: 10065711
2. Role of S'1 loop residues in the substrate specificities of pepsin A and chymosin.  |  Kageyama, T. 2004. Biochemistry. 43: 15122-30. PMID: 15568804
3. Processing of lysozyme at distinct loops by pepsin: a novel action for generating multiple antimicrobial peptide motifs in the newborn stomach.  |  Ibrahim, HR., et al. 2005. Biochim Biophys Acta. 1726: 102-14. PMID: 16137831
4. IgE binding to pepsin-digested food extracts.  |  Akkerdaas, JH., et al. 2005. Int Arch Allergy Immunol. 138: 203-8. PMID: 16205098
5. Effect of some phenolic compounds and beverages on pepsin activity during simulated gastric digestion.  |  Tagliazucchi, D., et al. 2005. J Agric Food Chem. 53: 8706-13. PMID: 16248575
6. Combining peptide modeling and capillary electrophoresis-mass spectrometry for characterization of enzymes cleavage patterns: recombinant versus natural bovine pepsin A.  |  Simó, C., et al. 2005. Anal Chem. 77: 7709-16. PMID: 16316180
7. Gastric antibacterial efficiency is different for pepsin A and C.  |  Schreiber, S., et al. 2006. Arch Microbiol. 184: 335-40. PMID: 16333616
8. Gastrointestinal digestion of Bet v 1-homologous food allergens destroys their mediator-releasing, but not T cell-activating, capacity.  |  Schimek, EM., et al. 2005. J Allergy Clin Immunol. 116: 1327-33. PMID: 16337467
9. The Controversy of Pepsinogen A/Pepsin A in Detecting Extra-Gastroesophageal Reflux.  |  Rao, YF., et al. 2023. J Voice. 37: 748-756. PMID: 34090740
10. Pepsin A in Tracheal Secretions From Patients Receiving Mechanical Ventilation.  |  Talbert, S., et al. 2021. Am J Crit Care. 30: 443-450. PMID: 34719715
11. Kinetic study of the action of bovine chymosin and pepsin A on bovine kappa-casein.  |  Carles, C. and Martin, P. 1985. Arch Biochem Biophys. 242: 411-6. PMID: 3933422
12. Use of Machine Learning Models to Predict Microaspiration Measured by Tracheal Pepsin A.  |  Bourgault, A., et al. 2025. Am J Crit Care. 34: 67-71. PMID: 39740967

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pepsin A Antibody (A-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Pepsin A Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pepsin A Antibody (A-10) Specifications

Alternate Product Name: Pepsin A

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 281-324

Epitope Target: Pepsin A

Epitope Species: human

Product Reactivity/Detection: Pepsin A and Pepsinogen A

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Pepsin A Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin