paxillin Antibody (C-10): sc-393149

paxillin Antibody (C-10) is a mouse monoclonal IgG1 kappa light chain antibody that specifically targets an epitope mapping between amino acids 59-89 near the N-terminus of human paxillin. Paxillin, also known as PXN, is a focal adhesion phosphoprotein localized to the cytoskeleton, playing a crucial role in cell adhesion, migration, and signal transduction pathways. Post-translational modifications of paxillin, such as phosphorylation on tyrosine and serine residues by kinases like focal adhesion kinase (FAK) and Src, are important because they regulate interactions with other proteins and modulate activity in cellular processes. Phosphorylation at specific sites, like Tyr118, influences paxillin′s ability to recruit structural and signaling molecules to focal adhesions, impacting cell morphology and motility. Additionally, paxillin can undergo ubiquitination and sumoylation, which affect stability and localization within the cell, further influencing function in signal transduction. During mitosis, paxillin levels are reduced through proteolytic downregulation, and alternatively phosphorylated on serine residues, highlighting a role in cell cycle regulation. anti-paxillin antibody (C-10) reacts with the α, β, and γ isoforms of paxillin from mouse, rat, and human origins in applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). paxillin (C-10) antibody is therefore a valuable tool for researchers studying the post-translational modifications and functions of paxillin in various cellular processes.

The preferred paxillin antibody is paxillin Antibody (B-2): sc-365379.