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p-Ser/Phosphoserine Anticorpo (16B4): sc-81514

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Fichas de dados
  • p-Ser/Phosphoserine Anticorpo 16B4é um anticorpo monoclonal produzido em camundongo IgM κ p-Ser/Phosphoserine anticorpo, citado em 95 publicações, fornecido em 50 µg/0.5 ml
  • sensibilizado contra a fosfoserina
  • recomendado para a detecção de a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine de mouse, rat, human e canine origem em
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de p-Ser/Phosphoserine (16B4): sc-81514.
  • Atualmente, ainda não concluímos a identificação do(s) reagente(s) de deteção secundário(s) preferido(s) para p-Ser/Phosphoserine Anticorpo (16B4). Este trabalho está em curso.

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Produtos auxiliares
Descricao
Ficha de dados e Protocolos
Informações de investigação

VEJA TAMBÉM

Adicionais p-Ser incluindo Anticorpos p-Thr e p-Tyr

O anticorpo p-Ser (16B4) é um anticorpo p-Ser monoclonal de camundongo IgM κ (também designado anticorpo de serina fosforilada, anticorpo de fosfosserina ou anticorpo de p-serina) que detecta a proteína p-Ser de origem de camundongo, rato, humana e canina por WB e IP. O anticorpo p-Ser (16B4) está disponível como anticorpo anti-p-Ser não conjugado. As proteínas quinases catalisam a fosforilação de resíduos de serina, treonina ou tirosina em substratos-alvo, fornecendo um mecanismo de controle para inúmeras vias de sinalização celular. Várias famílias de quinases fosforilam resíduos de serina e treonina em substratos-alvo, incluindo as famílias Raf, Rsk, ROCK, PAK, Ak e PKC de proteínas quinases de serina/treonina. A modificação de proteínas por fosforilação pode resultar em mudanças tridimensionais na estrutura da proteína e, assim, alterar sua atividade enzimática ou sua capacidade de interagir com outras proteínas. Os anticorpos direcionados à fosfoserina podem ser usados para a caracterização de proteínas com resíduos de serina fosforilados e para a elucidação de vias celulares que envolvem a fosforilação da serina.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Referencias do p-Ser/Phosphoserine Anticorpo (16B4):

  1. A estrutura primária da MEK, uma proteína quinase que fosforila o produto do gene ERK.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  2. A fosfoserina aminotransferase tem um sítio ativo conservado desde os micróbios até aos eucariotas superiores com pequenos desvios.  |  Singh, RK., et al. 2021. Protein Pept Lett. 28: 996-1008. PMID: 33588715
  3. A regulação negativa da fosfoserina fosfatase potencia os ambientes imunitários tumorais para melhorar a terapia de bloqueio do ponto de controlo imunitário.  |  Peng, ZP., et al. 2023. J Immunother Cancer. 11: PMID: 36849198
  4. As membranas de quitosano carregadas com fosfoserina promovem a regeneração óssea através da ativação de células estaminais endógenas.  |  Ke, Y., et al. 2023. Front Bioeng Biotechnol. 11: 1096532. PMID: 37034248
  5. Biossíntese e codificação genética de fosfoserina não hidrolisável em proteínas recombinantes em Escherichia coli.  |  Zhu, P., et al. 2023. Bio Protoc. 13: e4861. PMID: 37969748
  6. O excesso de fosfoserina-129 α-sinucleína induz o tráfico de vesículas sinápticas e defeitos de declusão numa sinapse de vertebrados.  |  Wallace, JN., et al. 2024. Mol Biol Cell. 35: ar10. PMID: 37991902
  7. Vias independentes de transdução de sinal da MAP-quinase humana definidas pelas isoformas MEK e MKK.  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
  8. Sítio de fosforilação da serina reguladora constitutiva mutante e putativa da MAP quinase cinase de mamíferos (MEK1).  |  Pagès, G., et al. 1994. EMBO J. 13: 3003-10. PMID: 8039496
  9. Fosforilação do domínio de transrepressão de c-Fos pela proteína quinase activada por mitogénio e pela quinase S6 ribossómica de 90-kDa.  |  Chen, RH., et al. 1993. Proc Natl Acad Sci U S A. 90: 10952-6. PMID: 8248197
  10. ROCK-I e ROCK-II, duas isoformas de serina/treonina quinase da proteína formadora de bobina enrolada associada a Rho em ratos.  |  Nakagawa, O., et al. 1996. FEBS Lett. 392: 189-93. PMID: 8772201
  11. O homólogo de Ste20 humano hPAK1 liga GTPases à via da quinase MAP JNK.  |  Brown, JL., et al. 1996. Curr Biol. 6: 598-605. PMID: 8805275
  12. Novos inibidores da fosfoserina fosfatase.  |  Hawkinson, JE., et al. 1997. Eur J Pharmacol. 337: 315-24. PMID: 9430431

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

p-Ser/Phosphoserine Anticorpo (16B4)

sc-81514
50 µg/0.5 ml
$316.00

Is there any possibility that p-Ser Antibody (16B4): sc-81514 does work for yeast samples as well...?

Asked by: yolyol
Thank you for your question. This antibody has only been tested in samples of mouse, rat, human and canine origin. It has not been tested in yeast species.
Answered by: Tech Support Europe
Date published: 2021-02-26

Hello, Which buffer is better to detect p-Ser antibody, skim-milk or 3-5% BSA? I immunoprecipitated phospho-protein and used 3% BSA in TBS-T buffer for blocking and 1st antibody but I couldn't get specific phosphorylation band.

Asked by: chohee0720
Thank you for your question. Please contact Technical Service directly for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2022-07-16

HI , I recently purchased this pserine antibody and I do see a band at 20kDa. why do i see a band arounf 20kda for pSerine?

Asked by: Dhanusha
Thank you for your question. As p-Ser Antibody (16B4): sc-81514 detects a broad range of serine-phosphorylated proteins in crude cell extracts, a signal at 20 kDa can respond to a large variety of different proteins of this molecular weight. The identity of this protein could be determined by assays such as MALDI-TOF.
Answered by: Tech Support Europe
Date published: 2020-07-14

The p-Ser Antibody (16B4) antibody is a monoclonal mouse IgM κ (kappa light chain). Still, you recommend the m-IgGκ BP-HRP: sc-516102 as a secondary antibody. But this a IgG-kappa specific ab. Would it still work?

Asked by: PeterPM
Thank you for your question. Yes, m-IgGκ BP-HRP: sc-516102 can detect any antibody that possesses a kappa light chain, including p-Ser Antibody (16B4): sc-81514, other IgM, and IgA antibodies.
Answered by: Technical Support Europe
Date published: 2019-05-06

Just wondering if your products of sc-81516 and sc-81514 recognize any of the phosphorylated S/T/Y, or they require certain residues beside these phosphorylated residues (like a certain motif).

Asked by: Zhyuhao
Thank you for your question. These antibodies both detect a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine. Additional information regarding the detected residues is not available.
Answered by: Tech Service
Date published: 2019-04-10

"preferring positively charged amino acids directly neighbored to phosphoserine". Does it mean that phosphoserine without neighboring positively charged amino acids will not be recognized by the 16B4 Ab ?

Asked by: Yo14
Thank you for your question. The antibody recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with pSK and pSP motifs strongly, recognizing substrates of MAP/SAP kinases and CDC kinases. Phosphoserine detection using an antibody generally is affected by the surrounding amino acids sequence, but it is possible that it can detect phosphoserine without neighboring + charged amino acids.
Answered by: Tech Service
Date published: 2018-08-09

Hi, is there any possibility to receive a sample from this antibody? Thanks for your answer.

Asked by: Constanze
Thank you for your question. We do not currently offer sample sizes for our p-Ser Antibody (16B4): sc-81514. This is a very popular and well cited antibody. If you purchase the antibody, please know that it is covered by our generous product warranty for one year from the date of purchase. If it does not work in any of the recommended applications, please contact our Technical Service Department and we can provide you with a replacement or refund.
Answered by: Technical Support
Date published: 2017-03-01

What is the expected molecular weight in Western blot when using p-Ser Antibody (16B4): sc-81514?

Asked by: cjMara
Thank you for your question. This antibody is recommended for detection of a broad range of serine-phosphorylated proteins. Therefore, the observed molecular weight is dependent upon which specific phosphorylated protein(s) you are evaluating with this antibody.
Answered by: Technical Support
Date published: 2016-12-22
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Rated 4 out of 5 by from p-Ser (16B4): sc-81514Western blot: Detecta multiples proteínas en extractos totales de células CRFK con 20 ug de proteína a una dilución de 1:500 y 1:1000 IP: Se utilizó 5 y 7 uL para inmunoprecipitar proteínas fosforiladas de células CRFK. Se utilizó la dilución de 1:500 para identificar las proteínas inmunoprecipitadas y sí se observan diversas bandas con diferentes pesos moleculares
Date published: 2024-04-01
Rated 5 out of 5 by from Works greatIt adequately detects phosphoserine antibodies. Even though it has some background, you can clean it up by more TBS T20 washes.
Date published: 2024-03-28
Rated 1 out of 5 by from 使えなかった一切バンドがでなかった。通常リン酸化抗体はブロードなバンドがみえるが、ひとつもバンドがみえなかった。
Date published: 2020-11-18
Rated 3 out of 5 by from Pretty good antibodyThe antibody works pretty well upon immunoprecipitation of the phosphorylated protein. On total cellular extract is too weak.
Date published: 2020-10-07
Rated 3 out of 5 by from Worked in WBphosphorylation was detected in Pull downed protein in WB
Date published: 2019-07-23
Rated 5 out of 5 by from Successful detectionSuccessful detection of Ser phosphorylation in immunoprecipitates
Date published: 2018-12-07
Rated 3 out of 5 by from P-Ser AntibodyCompared to other p-ser abs there was a lower background. Signal was much lower too. Tested in HeLa total lysates.
Date published: 2018-02-14
Rated 3 out of 5 by from Nice antibodyWe used this antibody for IP last month. It works well for some primary proteins. Overall a decent buy.
Date published: 2017-11-13
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p-Ser/Phosphoserine Anticorpo (16B4) is rated 4.0 out of 5 by 24.
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