Date published: 2025-9-8

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Neurofibromin Anticorpo (McNFn27a): sc-20017

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Fichas de dados
  • Neurofibromin Anticorpo McNFn27aé um anticorpo monoclonal produzido em camundongo IgG1 κ, citado em 4 publicações, fornecido em 200 µg/ml
  • ativado contra um péptido N-terminal de Neurofibromin de origem human
  • recomendado para a detecção de Neurofibromin de mouse, rat e human origem em
  • See Neurofibromin (H-12): sc-376886 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP e m-IgGκ BP-HRP são os reagentes de deteção secundários preferidos para Neurofibromin Anticorpo (McNFn27a) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com Neurofibromin Anticorpo (McNFn27a) (ver informações para encomenda abaixo).

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VEJA TAMBÉM

A neurofibromatose tipo 1 (NF1), ou neurofibromatose de von Reckinghausen, é um dos distúrbios autossômicos dominantes mais comuns em humanos. A análise de ligação inicial mapeou o gene NF1 no cromossomo 17. O transcrito previsto do NF1 codifica a proteína Neurofibromina de 2.818 aminoácidos, também designada como proteína relacionada ao NF1-GAP (NF1GRP). Por meio da análise da sequência, foi demonstrada a semelhança entre uma pequena região da Neurofibromina e membros da família genética Ras GAP. Funcionalmente, a Neurofibromina foi demonstrada por análise bioquímica envolvendo a hidrólise de RAS-GAP e complementação funcional em levedura para se assemelhar ainda mais à proteína GAP. A proteína Neurofibromina é expressa em níveis relativamente constantes em uma ampla gama de linhagens celulares e tecidos, incluindo cérebro, pulmão, fígado, rim, baço, músculo e cólon. Embora pouco se saiba sobre a função da Neurofibromina, a homologia com o domínio catalítico de proteínas com atividade GTPase sugere que a Neurofibromina também pode interagir in vivo com as proteínas Ras.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

Neurofibromin Anticorpo (McNFn27a)

sc-20017
200 µg/ml
$316.00

Pacote do Neurofibromin (McNFn27a): m-IgG Fc BP-HRP

sc-536640
200 µg Ab; 10 µg BP
$354.00

Pacote do Neurofibromin (McNFn27a): m-IgGκ BP-HRP

sc-533797
200 µg Ab; 40 µg BP
$354.00

Pacote do Neurofibromin (McNFn27a): m-IgG1 BP-HRP

sc-544725
200 µg Ab; 20 µg BP
$354.00

I am using Neurofibromin (McNFn27a): sc-20017 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from This antibody genuinely *does* work (even on WB)Hi. I am first author of one of the cited publications for this antibody (at least my pub still listed as 08/2025; Kahen, E. et al.) and I noticed someone else said they could not get signal on WB in their hands. I want to reassure that you really can get great WB's with this antibody for any human cell line, but it is a labor of love. Neurofibromin is a fairly large protein as you likely know, (typically considered 240 kd) often running somewhere around 210-240 kda range on a gel per MW markers. As I wrote in my methods, I had to use a tris-acetate gradient gel of 3-8% to capture NF1 and its putative isoform explicitly (Very painful gel to deal with; many gels were broken during troubleshooting). Getting the loading control was tricky because tubulin would run around the same range as a non-specific band. Alpha-actinin ran *very close* to a non-specific band so was not "publication worthy" but was good for testing, and was used, and others might benefit from this using alpha-actinin for loading control for NF1 if new antibodies more specific. I had to find perfect timing to be able to capture proper *actin* right at the bottom of the gel, which has far more specific WB antibodies for pub-worthy images. Note: Wet transfer 100% necessary to get all the NF1. Dry transfer would either over-transfer the actin or (most often) under-transfer the NF1. Very difficult to get sufficient NF1 on a membrane via dry-transfer without losing other bands for controls (and I was also really trying to get RAS on same gel). Honestly I think I legit tried dry transfer for a hilarious amount of time once and when I stained the gel out of curiosity, there was *still* so much protein in the NF1 region. I really tried many times with dry transfer- wet transfer only way to get anything useful. Then, I actually blocked membranes *overnight* in Odyssey blocking buffer. This Santa-Cruz NF1 antibody was then used at highest concentration recommended by manufacturer, again in Odyssey blocking buffer *overnight* (4degC on shaker). Very thorough washing in TBST (and I think I actually preferred lower tween 20 % but 10 min washes). I really wanted clean crisp neurofibromin showing two bands, as I did. And ultimately, I was able to get the result I wanted and reproduce it (though actin could really have looked a little prettier, but quantified accurately overall.) I suppose we should really be this detailed in our methods, explaining these sorts of things, but we tend not to be. Nonetheless, what I've described might help someone working with this antibody. Feel free to reach out via email and I will try to remember my best other than what I've written here.
Date published: 2025-08-02
Rated 1 out of 5 by from Not working for WB in our handsessentially no specific bands, nor non-specific bands.
Date published: 2019-05-29
Rated 4 out of 5 by from WB: Worked well in mouse spleen samplesWB: Worked well in mouse spleen samples. -SCBT Publication Review
Date published: 2015-06-18
Rated 5 out of 5 by from Published data for IF in human Schwann cellsPublished data for IF in human Schwann cells and CCF cells. -SCBT Publication Review
Date published: 2013-05-05
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Neurofibromin Anticorpo (McNFn27a) is rated 3.8 out of 5 by 4.
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