nestin Anticorpo (10c2) recomendado para a detecção de nestin de mouse, rat e human origem em

nestin Anticorpo (10c2): sc-23927

nestin Anticorpo (10c2) is rated 4.7 out of 5 by 27.
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Datasheets

    • Anti-nestin Anticorpo 10c2é um anticorpo monoclonal produzido em camundongo IgG1 κ nestin anticorpo, citado em 148 publicações, fornecido em 200 µg/ml
    • raised against a 150 amino acid epitope mapping near the C-terminus of human nestin
    • Anti-nestin Anticorpo (10c2) é recomendado para a detecção de nestin de mouse, rat e human origem em WB, IP, IF e IHC(P)
    • Anti-nestin Antibody (10c2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de nestin (10c2): sc-23927.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for nestin Antibody (10c2) for WB and IHC(P) applications. These reagents are now offered in bundles with nestin Antibody (10c2) (see ordering information below).
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I want evidence regarding Nestin in adult rodent amygdala if there's any image or reference for the same since I couldn't find anything related to that.

Asked by: Karishma
Thank you for your question. We have immunohistochemistry data for nestin in mouse cerebral cortex. We do not have any data or product citations specifically in amygdala for our nestin antibodies. This would have to be determined experimentally.
Answered by: Technical Support
Date published: 2020-05-11

Hi, I had a question regarding the unit size of one of your antibodies: sc-23927. Specifically, I was hoping to find out how much ug or mL I would be getting from one order of this product? Thanks! Eric

Asked by: turdyturtle
Thank you for your question. This antibody, nestin (10c2): sc-23927, is provided as 200 µg in 1 mL.
Answered by: Technical Support
Date published: 2017-07-26

I am setting up a new lab and shopping for a bunch of antibodies. I am looking for an alternative for discontinued Nestin (goat). Your nestin Antibody (10c2): sc-23927 looks promsing. Would you please providing any sample? This help us choose a ventor.

Asked by: Michael1
Thank you for your question. For a limited time, we are currently offering free 50µg samples of our mouse monoclonal antibodies, including, nestin (10c2): sc-23927. This antibody has been validated to work in WB, IP and IHC(P) with mouse, rat and human samples. Please contact our Technical Service Department to request a sample. Please call us toll-free at (800) 457-3801 option 2, email [email protected], or use the live chat on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2018-11-08

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-22
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Rated 4 out of 5 by from Nice specificity in IF Ottimo per neural stem cells e brain tumour stem cells
Date published: 2019-04-09
Rated 4 out of 5 by from Good antibody This antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from It works good in ONPC WB We tested in western blot, 40ug protein, 7% de Acrylamide (1:200) and it worked good in our samples of olfactory neuroepitellium cells (human).
Date published: 2018-10-01
Rated 5 out of 5 by from Sehr Gute Ergebnisse Sehr gute Ergebnisse bei Western Blot und auch bei Immunoprecipitation.
Date published: 2018-09-21
Rated 5 out of 5 by from Great antibody for IHC We used this antibody on NPCs, and it worked great.
Date published: 2018-07-19
Rated 3 out of 5 by from Works OK This antibody stains fair in neuronal cell derived from human somatic cells.
Date published: 2017-12-21
Rated 5 out of 5 by from Good Antibody Tested this antibody by 0.5% Triton X-100 permeabilization for 5 min and antibody diluted at 1:200 then incubated overnight at 4C. Saw nice clean nestin fibers in NSCs.
Date published: 2017-12-05
Rated 3 out of 5 by from Minimal reaction product I requested the trial-sized sample of this antibody for IHC(P) and WB. Unfortunately, reaction product on sections of human spinal cord was minimal. No crisp, distinct bands appeared on WB with human spinal cord lysate. Perhaps the autopsied tissues are to blame (e.g. autolysis), and each application could be improved with a tweaked protocol.
Date published: 2017-09-21
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