MTDH Antibody (2F11C3D4): sc-517220

MTDH Antibody (2F11C3D4) is a mouse monoclonal IgG1 antibody that detects the MTDH protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-MTDH antibody (2F11C3D4) is available as a non-conjugated format. MTDH, or metastasis adhesion protein, also known as astrocyte elevated gene-1 protein (AEG1) or lysine-rich CEACAM1 co-isolated protein (LYRIC), is a 582 amino acid single-pass membrane protein that plays a critical role in cancer progression. MTDH is primarily localized to the endoplasmic reticulum membrane, but can translocate from the cytoplasm to the nucleus in response to tumor necrosis factor-alpha (TNFα), highlighting involvement in cellular signaling pathways. MTDH interacts with various proteins, including transcription factor NFκB, which MTDH activates to promote anchorage-independent growth of immortalized astrocytes and melanocytes, essential processes in tumor development. Additionally, MTDH enhances the seeding of tumor cells to the endothelium of target organs, making MTDH a significant player in metastasis. MTDH expression is notably elevated in several cancers and is found at high levels in tissues such as the heart, skeletal muscle, tongue, and small intestine, underscoring importance in both normal physiology and disease states.

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MTDH Antibody (2F11C3D4) References:

1. Cloning and characterization of HIV-1-inducible astrocyte elevated gene-1, AEG-1.  |  Kang, DC., et al. 2005. Gene. 353: 8-15. PMID: 15927426
2. Activation of the nuclear factor kappaB pathway by astrocyte elevated gene-1: implications for tumor progression and metastasis.  |  Emdad, L., et al. 2006. Cancer Res. 66: 1509-16. PMID: 16452207
3. Astrocyte elevated gene-1: recent insights into a novel gene involved in tumor progression, metastasis and neurodegeneration.  |  Emdad, L., et al. 2007. Pharmacol Ther. 114: 155-70. PMID: 17397930
4. Knockdown of astrocyte-elevated gene-1 inhibits prostate cancer progression through upregulation of FOXO3a activity.  |  Kikuno, N., et al. 2007. Oncogene. 26: 7647-55. PMID: 17563745
5. Molecular basis of nuclear factor-kappaB activation by astrocyte elevated gene-1.  |  Sarkar, D., et al. 2008. Cancer Res. 68: 1478-84. PMID: 18316612
6. LYRIC/AEG-1 overexpression modulates BCCIPalpha protein levels in prostate tumor cells.  |  Ash, SC., et al. 2008. Biochem Biophys Res Commun. 371: 333-8. PMID: 18440304
7. Astrocyte elevated gene-1 is a novel prognostic marker for breast cancer progression and overall patient survival.  |  Li, J., et al. 2008. Clin Cancer Res. 14: 3319-26. PMID: 18519759
8. MTDH activation by 8q22 genomic gain promotes chemoresistance and metastasis of poor-prognosis breast cancer.  |  Hu, G., et al. 2009. Cancer Cell. 15: 9-20. PMID: 19111877

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MTDH Antibody (2F11C3D4) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-MTDH Antibody (2F11C3D4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MTDH Antibody (2F11C3D4) Specifications

Alternate Product Name: MTDH

Product Clone Name: 2F11C3D4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 378-579

Epitope Target: MTDH

Epitope Species: human

Product Reactivity/Detection: MTDH

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 5% glycerol

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for MTDH Antibody (2F11C3D4): N/A