GAPDH (0411): sc-47724. Análise fluorescente por western blot da expressão de GAPDH em lisados de células inteiras de Jurkat (A), HeLa (B), K-562 (C), BJAB (D) e IMR-32 (E). Bloqueado com Reagente de Bloqueio UltraCruz®: sc-516214. Reagente de deteção utilizado: m-IgG1 BP-CFL 647: sc-533664.
GAPDH (0411): sc-47724. Análise fluorescente por western blot da expressão de GAPDH em lisados de células inteiras de Jurkat (A), HeLa (B), K-562 (C), BJAB (D) e IMR-32 (E). Bloqueado com Reagente de Bloqueio UltraCruz®: sc-516214. Reagente de deteção utilizado: m-IgG1 BP-CFL 647: sc-533664.
mouse IgG1 binding protein (m-IgG1 BP) conjugated to CruzFluor™ 647 (CFL 647)
fornecidos a 100 µg em 0.5 ml de volume
CruzFluor™ 647 (CFL 647) conjugated secondaries are similar to Alexa Fluor® 647 and Cy5® fluorescent dyes, offering comparable photostability and the ability to resist protein quenching
Excitation of 654 nm, Emission of 669 nm
Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
Substituto altamente recomendado para os anticorpos secundários convencionais IgG anti-camundongo para RGB Western blotting (WB [RGB]), Imunofluorescência (IF) e Citometria de Fluxo (FCM)
Suitable for binding to most, but not all, mouse IgG1 immunoglobulins; not suitable for use with mouse monoclonal IgG2a, IgG2b, IgG3, IgM, IgA or IgE antibodies. May slightly cross react with mouse IgG2b and goat IgG antibodies
Not cross-reactive with human, rat or rabbit IgG antibodies
Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
LINKS RÁPIDOS
Descricao
Ficha de dados e Protocolos
Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.
Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA