MKP-3 Antibody (G-11): sc-137245

MKP-3 Antibody (G-11) is a mouse monoclonal IgG1 antibody that detects MKP-3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MKP-3, a member of the dual specificity phosphatase family, plays a crucial role in regulating mitogen-activated protein (MAP) kinase signaling pathways by specifically dephosphorylating critical phosphothreonine and phosphotyrosine residues within ERK family members. This regulation is vital as it helps maintain cellular homeostasis and modulates responses to various stimuli, including growth factors and stress signals. MKP-3 expression is particularly prominent in the heart and pancreas, highlighting its importance in tissues that require precise control of signaling pathways for proper function. Additionally, MKP-3 is encoded by a gene located on chromosome 12q22-q23, producing a 381 amino acid protein that is essential for the negative regulation of MAP kinases, thereby influencing a range of physiological processes. Anti-MKP-3 antibody (G-11) is an invaluable tool for researchers studying the intricate dynamics of MAP kinase signaling and its implications in health and disease.

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MKP-3 Antibody (G-11) References:

1. MKP3 negatively modulates PDGF-induced Akt and Erk5 phosphorylation as well as chemotaxis.  |  Razmara, M., et al. 2012. Cell Signal. 24: 635-40. PMID: 22100392
2. Caspase-3 cleavage of DUSP6/MKP3 at the interdomain region generates active MKP3 fragments that regulate ERK1/2 subcellular localization and function.  |  Cejudo-Marín, R., et al. 2012. J Mol Biol. 420: 128-38. PMID: 22504224
3. Ischemia/reperfusion-induced MKP-3 impairs endothelial NO formation via inactivation of ERK1/2 pathway.  |  Yang, D., et al. 2012. PLoS One. 7: e42076. PMID: 22848708
4. Mitogen-activated protein kinase phosphatase 3 (MKP-3)-deficient mice are resistant to diet-induced obesity.  |  Feng, B., et al. 2014. Diabetes. 63: 2924-34. PMID: 24722245
5. MKP-3 regulates PDGF-BB effects and MAPK activation in meningioma cells.  |  Johnson, MD., et al. 2015. J Clin Neurosci. 22: 752-7. PMID: 25698542
6. Overexpression of Mitogen-activated protein kinase phosphatase-3 (MKP-3) reduces FoxO1 phosphorylation in mice hypothalamus.  |  Rodrigues, BA., et al. 2017. Neurosci Lett. 659: 14-17. PMID: 28866049
7. MAP Kinase Phosphatase 3 (MKP3) Preserves Norepinephrine Transporter Activity by Modulating ERK1/2 Kinase-Mediated Gene Expression.  |  Mortensen, OV., et al. 2017. Front Cell Neurosci. 11: 253. PMID: 28878626
8. MKP-3 suppresses LPS-induced inflammatory responses in HUVECs via inhibition of p38 MAPK/NF-κB pathway.  |  Unenkhuu, B., et al. 2021. Anim Cells Syst (Seoul). 25: 235-244. PMID: 34408812
9. Cynarin attenuates LPS-induced endothelial inflammation via upregulation of the negative regulator MKP-3.  |  Kim, DB., et al. 2022. Anim Cells Syst (Seoul). 26: 119-128. PMID: 35784390
10. Hepatocentric Leptin Signaling Modulates Gluconeogenesis via MKP-3.  |  Gamarra, JR. and Haeusler, RA. 2022. Cell Mol Gastroenterol Hepatol. 14: 1166-1167. PMID: 36088958

The preferred MKP-3 antibody is MKP-3 Antibody (F-12): sc-377070.