LAMP-2 Antibody (5K54): sc-71490

LAMP-2 Antibody (5K54) is a rat monoclonal IgG2a antibody raised against glycoprotein fractions purified from the BALB/c mouse embryo 3T3 cell line, with species reactivity in mouse. LAMP-2 monoclonal antibody (5K54) is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). LAMP-2, also known as CD107B, is a glycosylated type I transmembrane protein predominantly localized to the lysosomal membrane. Extensive glycosylation stabilizes LAMP-2, protects the lysosomal membrane from hydrolytic enzymes, and facilitates autophagy by mediating the fusion of autophagosomes with lysosomes. LAMP-2 is synthesized in the endoplasmic reticulum, undergoes glycosylation in the Golgi apparatus, and travels to lysosomes via a pathway distinct from clathrin-coated vesicles. Dysregulation of LAMP-2 expression or modifications has been implicated in lysosomal storage disorders such as Danon disease, highlighting LAMP-2′s importance in lysosomal function and cellular homeostasis. Altered LAMP-2 activity has been linked to tumor invasion and metastasis due to roles in cellular adhesion and proteolytic activity. Anti-LAMP-2 antibody (5K54) serves as an essential tool for exploring post-translational modifications and biological functions of LAMP-2 in health and disease.

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LAMP-2 Antibody (5K54) References:

1. Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis.  |  Lichter-Konecki, U., et al. 1999. Differentiation. 65: 43-58. PMID: 10448712
2. Arrested maturation of Neisseria-containing phagosomes in the absence of the lysosome-associated membrane proteins, LAMP-1 and LAMP-2.  |  Binker, MG., et al. 2007. Cell Microbiol. 9: 2153-66. PMID: 17506821
3. Immunohistochemistry of LAMP-2 and adipophilin for phospholipidosis in liver and kidney in ketoconazole-treated mice.  |  Asaoka, Y., et al. 2013. Exp Toxicol Pathol. 65: 817-23. PMID: 23276623
4. Lysosome-associated protein 1 (LAMP-1) and lysosome-associated protein 2 (LAMP-2) in a larger family carrier of Fabry disease.  |  Pereira, EM., et al. 2014. Gene. 536: 118-22. PMID: 24334114
5. Intermittent and graded exercise effects on NK cell degranulation markers LAMP-1/LAMP-2 and CD8+CD38+ in chronic fatigue syndrome/myalgic encephalomyelitis.  |  Broadbent, S. and Coutts, R. 2017. Physiol Rep. 5: PMID: 28275109
6. Surface LAMP-2 Is an Endocytic Receptor That Diverts Antigen Internalized by Human Dendritic Cells into Highly Immunogenic Exosomes.  |  Leone, DA., et al. 2017. J Immunol. 199: 531-546. PMID: 28607115
7. Lysosome-associated membrane protein 2 (LAMP-2) expression induced by miR-194-5p downregulation contributes to sunitinib resistance in human renal cell carcinoma cells.  |  Yumioka, T., et al. 2018. Oncol Lett. 15: 893-900. PMID: 29399154
8. Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan.  |  Carlsson, SR., et al. 1988. J Biol Chem. 263: 18911-9. PMID: 3143719
9. Danon Disease-Associated LAMP-2 Deficiency Drives Metabolic Signature Indicative of Mitochondrial Aging and Fibrosis in Cardiac Tissue and hiPsc-Derived Cardiomyocytes.  |  Del Favero, G., et al. 2020. J Clin Med. 9: PMID: 32751926
10. Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells.  |  Sarafian, V., et al. 1998. Int J Cancer. 75: 105-11. PMID: 9426697

The preferred LAMP-2 antibody is LAMP-2 Antibody (H4B4): sc-18822.