LAMP-2 Antibody (4E152): sc-71491

LAMP-2 Antibody (4E152) is a rat monoclonal IgG antibody raised against purified mouse liver lysosomal membranes, with species reactivity specific to mouse. LAMP-2 (4E152) monoclonal antibody is validated for western blotting (WB) and immunoprecipitation (IP). LAMP-2, also known as CD107B, is a glycosylated type I transmembrane protein localized to lysosomal membranes, where LAMP-2 is critical for lysosomal function and cellular homeostasis. LAMP-2 plays a key role in autophagy by facilitating the fusion of autophagosomes with lysosomes, enabling the degradation and recycling of cellular components. LAMP-2 also maintains lysosomal integrity and acidity, protecting the membrane from autodigestion by lysosomal enzymes. LAMP-2 is synthesized in the endoplasmic reticulum, glycosylated in the Golgi apparatus, and transported to lysosomes via a pathway distinct from clathrin-coated vesicles. LAMP-2′s functional importance extends to processes like cellular adhesion and intracellular trafficking, and dysregulation of LAMP-2 is linked to lysosomal storage disorders such as Danon disease. By studying LAMP-2, researchers can gain insights into lysosomal dynamics, autophagy, and their implications in health and disease. Anti-LAMP-2 antibody (4E152) is a valuable tool for investigating LAMP-2′s role in maintaining lysosomal and cellular function.

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LAMP-2 Antibody (4E152) References:

1. Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice.  |  Tanaka, Y., et al. 2000. Nature. 406: 902-6. PMID: 10972293
2. Role of LAMP-2 in lysosome biogenesis and autophagy.  |  Eskelinen, EL., et al. 2002. Mol Biol Cell. 13: 3355-68. PMID: 12221139
3. The polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2. Localization on the peptide backbones.  |  Carlsson, SR. and Fukuda, M. 1990. J Biol Chem. 265: 20488-95. PMID: 2243102
4. The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins.  |  Cha, Y., et al. 1990. J Biol Chem. 265: 5008-13. PMID: 2318880
5. Property of lysosomal storage disease associated with midbrain pathology in the central nervous system of Lamp-2-deficient mice.  |  Furuta, A., et al. 2015. Am J Pathol. 185: 1713-23. PMID: 25998250
6. Exendin-4 impairs the autophagic flux to induce apoptosis in pancreatic acinar AR42J cells by down-regulating LAMP-2.  |  Li, Z., et al. 2018. Biochem Biophys Res Commun. 496: 294-301. PMID: 29317206
7. Multiple mRNAs encode the avian lysosomal membrane protein LAMP-2, resulting in alternative transmembrane and cytoplasmic domains.  |  Hatem, CL., et al. 1995. J Cell Sci. 108 (Pt 5): 2093-100. PMID: 7657727
8. The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes.  |  Sawada, R., et al. 1993. J Biol Chem. 268: 9014-22. PMID: 8517882
9. Sorting of lysosomal membrane glycoproteins lamp-1 and lamp-2 into vesicles distinct from mannose 6-phosphate receptor/gamma-adaptin vesicles at the trans-Golgi network.  |  Karlsson, K. and Carlsson, SR. 1998. J Biol Chem. 273: 18966-73. PMID: 9668075
10. The extent of polylactosamine glycosylation of MDCK LAMP-2 is determined by its Golgi residence time.  |  Nabi, IR. and Dennis, JW. 1998. Glycobiology. 8: 947-53. PMID: 9675228

The preferred LAMP-2 antibody is LAMP-2 Antibody (H4B4): sc-18822.