Date published: 2025-9-8

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LAMP1 Anticorpo (1D4B): sc-19992

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Fichas de dados
  • LAMP1 Anticorpo 1D4Bé um anticorpo monoclonal produzido em rato IgG2a, citado em 280 publicações, fornecido em 200 µg/ml
  • elevada contra membranas de células de cultura de tecidos de fibroblastos de embriões de ratinho NIH/3T3
  • Anti-LAMP1 Anticorpo (1D4B) é recomendado para a detecção de LAMP-1 de mouse, rat e human origem em WB, IP, IF e FCM
  • Anticorpo anti-LAMP1 O anticorpo anti-LAMP1 (1D4B) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 405 para IF, IHC(P) e FCM
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de LAMP1 (1D4B): sc-19992.
  • Atualmente, ainda não concluímos a identificação do(s) reagente(s) de deteção secundário(s) preferido(s) para LAMP1 Anticorpo (1D4B). Este trabalho está em curso.

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VEJA TAMBÉM

O anticorpo LAMP-1 (1D4B) é um anticorpo monoclonal IgG2a de rato que detecta LAMP-1 em amostras de camundongo, rato e humano através de aplicações como western blotting (WB), imunoprecipitação (IP), imunofluorescência (IF) e citometria de fluxo (FCM). O anticorpo anti-LAMP-1 (1D4B) está disponível em formas não conjugadas e em várias formas conjugadas, incluindo agarose, peroxidase de rábano (HRP), ficoeritrina (PE), isotiocianato de fluoresceína (FITC) e vários conjugados Alexa Fluor®. A LAMP-1, também conhecida como CD107a, é uma proteína de membrana glicosilada de tipo I localizada principalmente na membrana lisossómica, onde a LAMP-1 desempenha um papel crucial na manutenção da integridade e função lisossómica. A localização correta da LAMP-1 ajuda a regular a acidez lisossomal e protege as membranas lisossomais da autodigestão, o que é essencial para a homeostase celular. O LAMP-1 está envolvido em vários processos celulares, incluindo a adesão celular e a modulação de respostas imunitárias, particularmente em plaquetas activadas, onde a expressão de LAMP-1 é suprarregulada. Este suprarregulado contribui para as propriedades adesivas e pró-trombóticas destas células, realçando a importância do LAMP-1 tanto na fisiologia normal como em condições patológicas, como as doenças de armazenamento lisossómico, em que a expressão do LAMP-1 está acentuadamente elevada.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Referencias do LAMP1 Anticorpo (1D4B):

  1. Morfologia e função lisossomal normais em ratinhos deficientes em LAMP-1.  |  Andrejewski, N., et al. 1999. J Biol Chem. 274: 12692-701. PMID: 10212251
  2. Os oligossacáridos ligados à asparagina protegem Lamp-1 e Lamp-2 da proteólise intracelular.  |  Kundra, R. and Kornfeld, S. 1999. J Biol Chem. 274: 31039-46. PMID: 10521503
  3. Tráfego de colesterol perturbado mas função proteolítica normal em fibroblastos com deficiência dupla de LAMP-1/LAMP-2.  |  Eskelinen, EL., et al. 2004. Mol Biol Cell. 15: 3132-45. PMID: 15121881
  4. Transthyretin interage com a proteína de membrana associada ao lisossoma (LAMP-1) em circulação.  |  Chang, MH., et al. 2004. Biochem J. 382: 481-9. PMID: 15200388
  5. As proteínas de membrana associadas ao lisossoma das células dendríticas (LAMP) e as quimeras LAMP-1-HIV-1 gag têm vias de tráfico celular distintas e preparam as respostas das células T e B para um repertório diversificado de epítopos.  |  Arruda, LB., et al. 2006. J Immunol. 177: 2265-75. PMID: 16887987
  6. Maturação interrompida de fagossomas contendo Neisseria na ausência das proteínas de membrana associadas ao lisossoma, LAMP-1 e LAMP-2.  |  Binker, MG., et al. 2007. Cell Microbiol. 9: 2153-66. PMID: 17506821
  7. Identificação e caraterização da LAMP-1 como uma glicoproteína de superfície plaquetária dependente da ativação.  |  Febbraio, M. and Silverstein, RL. 1990. J Biol Chem. 265: 18531-7. PMID: 2211717
  8. Glicoproteínas de membrana lisossomal: propriedades de LAMP-1 e LAMP-2.  |  Chen, JW., et al. 1986. Biochem Soc Symp. 51: 97-112. PMID: 3101702
  9. As glicoproteínas de membrana lisossómica Lamp-1 e Lamp-2 estão presentes em organelos mobilizáveis, mas estão ausentes dos grânulos azurófilos dos neutrófilos humanos.  |  Dahlgren, C., et al. 1995. Biochem J. 311 (Pt 2): 667-74. PMID: 7487911

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

LAMP1 Anticorpo (1D4B)

sc-19992
200 µg/ml
$316.00

LAMP1 Anticorpo (1D4B) AC

sc-19992 AC
500 µg/ml, 25% agarose
$416.00

LAMP1 Anticorpo (1D4B) HRP

sc-19992 HRP
200 µg/ml
$316.00

LAMP1 Anticorpo (1D4B) FITC

sc-19992 FITC
200 µg/ml
$330.00

LAMP1 Anticorpo (1D4B) PE

sc-19992 PE
200 µg/ml
$343.00

LAMP1 Anticorpo (1D4B) Alexa Fluor® 488

sc-19992 AF488
200 µg/ml
$357.00

LAMP1 Anticorpo (1D4B) Alexa Fluor® 546

sc-19992 AF546
200 µg/ml
$357.00

LAMP1 Anticorpo (1D4B) Alexa Fluor® 594

sc-19992 AF594
200 µg/ml
$357.00

LAMP1 Anticorpo (1D4B) Alexa Fluor® 647

sc-19992 AF647
200 µg/ml
$357.00

LAMP1 Anticorpo (1D4B) Alexa Fluor® 680

sc-19992 AF680
200 µg/ml
$357.00

LAMP1 Anticorpo (1D4B) Alexa Fluor® 790

sc-19992 AF790
200 µg/ml
$357.00

LAMP1 Anticorpo (1D4B) Alexa Fluor® 405

sc-19992 AF405
200 µg/ml
$357.00

O anticorpo em questão, está validado em mouse brain? (LAMP-1 ANTIBODY 1D4B)

Asked by: 1D4B
Thank you for your question. LAMP1 Antibody (1D4B): sc-19992 is recommended for detection of LAMP-1 of mouse, rat and human origin by WB, IP, IF and FCM. It can be used on mouse brain samples.
Answered by: Tech Support Europe
Date published: 2025-03-21

Hi! Is it possible to recognize the Lamp1 1D4B 488 with a secondary antibody or is the Alexa Fluor disturbing this process? Thank you.

Asked by: Karletz
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

is this antibody still available? Can I order it from thermofisher as a supplier? Thank you!

Asked by: Jing2
Thank you for your question. This LAMP-1 antibody is currently available. Please contact our Customer Care Department for further support.
Answered by: Technical Support
Date published: 2020-07-16

Hello I'm a researcher at City of Hope Medical Center. We are curious if the monoclonal antibody is targeting the glycan structure on LAMP-1 or the primary structure of the protein. Please let me know at your earliest convenience. Thx.

Asked by: Tong95
Thank you for your question. This antibody was raised against raised against NIH/3T3 mouse embryo fibroblast tissue culture cell membranes. The exact binding epitope has not been mapped. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-02-18

Has sc-19992 been used for Western blots with the Licor system? I have not been able to detect Lamp1 in 293T/17 lysates with 1D4B, but have always been able to see Lamp1 with H4A3 on our Odyssey.

Asked by: Anonymous1
Thank you for your question. We have not specifically tested this LAMP-1 Antibody (1D4B): sc-19992 using the Licor Odyssey system for detection. But the antibody does work for Western blot, so we would not expect any difficulty using it in a system with infrared detection reagents. LAMP-1 Antibody (1D4B) is a rat monoclonal IgG2a, while the other clone, (H4A3) is a mouse monoclonal IgG1. Please check to see if the Licor-Odyssey secondary antibodies you are using are compatible with detection of both of these host species and isotypes. Please contact our Technical Service Department and we would be happy to assist you with troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-07-12

I would like to get anti-rat Lamp-1 for IF. Are there papers using this antibody for IF? Could I get a sample?

Asked by: AAABBB
Thank you for your question. This particular LAMP-1 Antibody (1D4B): sc-19992 has been cited in 17 papers that we are aware of for use in IF. For example, PMID's: 15998735 and 23600861. For a complete list, you can search by product catalog number and research application (IF) on our website. See the link below for more information. https://www.scbt.com/scbt/misc/citationResults.jsp?_requestid=7296121 We do not currently offer a sample size of this antibody. This is an excellent well-established antibody and it is covered by our product warranty if you purchase it and have any trouble using it we can replace it or refund your purchase within one year of purchase. We do offer a 10 µg sample size of an alternative clone, LAMP-1 Antibody (H4A3): sc-20011. A link to the product page is below. This antibody is also suitable for IF on rat samples. Please contact our Technical Service Department if you would like to request a sample. https://www.scbt.com/scbt/product/lamp-1-antibody-h4a3?requestFrom=search
Answered by: Technical Support
Date published: 2017-02-23

What is the epitope recognized by LAMP-1 (1D4B)?

Asked by: cjMara
Thank you for your question. LAMP-1 Antibody (1D4B) is a rat monoclonal raised against NIH/3T3 mouse embryo fibroblast tissue culture cell membranes. The exact epitope recognized by this antibody has not been determined.
Answered by: Technical Support
Date published: 2016-12-22
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Rated 5 out of 5 by from Beautiful stainingBright signal was detected in immunocytostaining of cultured mouse neural cells.
Date published: 2024-03-29
Rated 4 out of 5 by from Good for IFImmunofluorescenceにおいてリソソームのマーカーとして使用しました。きれいにリソソームを染色することができました。
Date published: 2020-08-24
Rated 5 out of 5 by from works for WB and ICCWe got a sample and then purchased a full size. Antibody is looking fine both for Western blot and ICC
Date published: 2019-04-16
Rated 5 out of 5 by from Great antibodyUsed for western blotting. Dilution 1:1000, 1% skimmed milk in TBST, HT22 cell lysate (20ug)
Date published: 2019-03-25
Rated 5 out of 5 by from Good antibody!I use this for IF analyses in the Hinck lab, and I see great detection. I have also used the conjugated form (680), and it works great in combination with other SCBT conjugated antibodies.
Date published: 2018-05-09
Rated 5 out of 5 by from Very good Ab for WBI got free sample of this Ab from Santa Cruz and Tested it on mouse liver tissue and works very well. One single band as expected.
Date published: 2018-04-26
Rated 5 out of 5 by from My Review for LAMP-1 Antibody (1D4B)Excellent antibody for western blotting ( 1:500 in 5% BSA) and Immunofluorescence ( 1:50 on methanol fixed cells/ FFPE epidermis ) in primary mouse keratinocytes and mouse epidermal tissue
Date published: 2018-03-18
Rated 5 out of 5 by from great antibodyI used this antibody for mouse heart frozen tissue staining. It worked very beautiful.
Date published: 2018-01-22
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LAMP1 Anticorpo (1D4B) is rated 4.5 out of 5 by 22.
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