Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
JMJD2A Double Nickase Plasmid (m): sc-432966-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JMJD2A Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- JMJD2A Double Nickase Plasmid (m) and JMJD2A Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Kdm4a. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JMJD2A Antibody (D-9): sc-271210
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JMJD2A Double Nickase Plasmid (m) | sc-432966-NIC | 20 µg | $410.00 | |||
JMJD2A Double Nickase Plasmid (m2) | sc-432966-NIC-2 | 20 µg | $410.00 |
Mouse Kdm4a encodes the lysine demethylase JMJD2A (KDM4A), a Jumonji C domain–containing enzyme that removes repressive histone marks such as H3K9me3/me2 and H3K36me3/me2 to regulate chromatin accessibility and transcriptional programs. JMJD2A participates in epigenetic control of cell-cycle progression, DNA replication and repair, and lineage-specific gene expression, integrating with chromatin remodeling and transcription factor networks. Through its effects on heterochromatin organization and genome stability, Kdm4a is frequently studied in pathways linked to oncogenic transcriptional states, aberrant proliferation, and stress responses. Dysregulated JMJD2A activity has been associated with altered differentiation and transformation phenotypes, supporting its use as a mechanistic node in disease-relevant epigenomics research.
JMJD2A Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Kdm4a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Kdm4a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Kdm4a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Kdm4a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.