Intra-acrosomal protein Antibody (Ds-2): sc-51659

Intra-acrosomal protein Antibody (Ds-2) is a mouse monoclonal IgG antibody that detects the Intra-acrosomal protein of canine origin by western blotting (WB) and immunofluorescence (IF). Anti-Intra-acrosomal protein antibody (Ds-2) is available as a non-conjugated format. Intra-acrosomal protein, also referred to as acrosomal vesicle protein 1 and acrosomal protein SP-10, plays a crucial role in spermatogenesis as a testis-specific differentiation antigen that forms within the acrosomal vesicle. Intra-acrosomal protein is integral to the acrosomal membranes and matrix of mature sperm, facilitating essential processes such as sperm-zona binding and penetration, which are vital for successful fertilization. The gene encoding Intra-acrosomal protein consists of four exons, and through alternative splicing, generates 11 distinct transcripts that produce protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most prevalent, accounting for 53-72% of total Intra-acrosomal protein messages, while the second largest transcript comprises 15-32%. The remaining transcripts contribute to a smaller fraction of total message, indicating a complex regulation of expression that may involve cryptic splicing and exon skipping phenomena. Given its significant role in reproductive biology, Intra-acrosomal protein is also being explored as a potential immunogen for contraceptive vaccines in humans, highlighting its importance beyond canine biology.

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Intra-acrosomal protein Antibody (Ds-2) References:

1. Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro.  |  Peknicova, J., et al. 2001. Theriogenology. 56: 211-23. PMID: 11480614
2. Purification and microsequencing of the intra-acrosomal protein SP-10. Evidence that SP-10 heterogeneity results from endoproteolytic processes.  |  Herr, JC., et al. 1992. Biol Reprod. 47: 11-20. PMID: 1637938
3. Cloning and sequencing of cDNAs coding for the human intra-acrosomal antigen SP-10.  |  Wright, RM., et al. 1990. Biol Reprod. 42: 693-701. PMID: 1693291
4. Assignment of the gene for human intra-acrosomal protein SP-10 to the p12----q13 region of chromosome 11.  |  Herr, JC., et al. 1991. J Androl. 12: 281-7. PMID: 1765564
5. Tissue specificity of the acrosomal protein SP-10: a contraceptive vaccine candidate molecule.  |  Freemerman, AJ., et al. 1994. Biol Reprod. 50: 615-21. PMID: 8167233
6. Refinement of the localization of the gene for human intraacrosomal protein SP-10 (ACRV1) to the junction of bands q23-->q24 of chromosome 11 by nonisotopic in situ hybridization.  |  Golden, WL., et al. 1993. Genomics. 18: 446-9. PMID: 8288254
7. Cloning and characterization of the gene coding for the human acrosomal protein SP-10.  |  Wright, RM., et al. 1993. Biol Reprod. 49: 316-25. PMID: 8373955
8. Stage-specific detection of mRNA for the sperm antigen SP-10 in human testes.  |  Kurth, BE., et al. 1993. Anat Rec. 236: 619-25. PMID: 8379586
9. Inhibition of bovine fertilization in vitro by antibodies to SP-10.  |  Coonrod, SA., et al. 1996. J Reprod Fertil. 107: 287-97. PMID: 8882296

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Intra-acrosomal protein Antibody (Ds-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Intra-acrosomal protein Antibody (Ds-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Intra-acrosomal protein Antibody (Ds-2) Specifications

Alternate Product Name: Intra-acrosomal protein

Product Clone Name: Ds-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: Intra-acrosomal protein

Epitope Species: canine

Product Reactivity/Detection: intra-acrosomal proteins (92 and 42 kDa)

Species Reactivity/Detection: canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF

Vialing Information: Each vial contains 100 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Intra-acrosomal protein Antibody (Ds-2): N/A