Date published: 2025-9-8

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IL-1 beta/IL1B Anticorpo (E7-2-hIL1β): sc-32294

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Fichas de dados
  • IL-1 beta/IL1B Anticorpo E7-2-hIL1βé um anticorpo monoclonal produzido em camundongo IgG2b κ, citado em 133 publicações, fornecido em 200 µg/ml
  • Produzido contra recombinante IL-1β de human origem
  • IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) é recomendado para a detecção de IL-1β of human origin by WB, IP, IF and IHC(P)
  • Anticorpo anti-IL-1 beta/IL1B O anticorpo anti-IL-1 beta/IL1B (E7-2-hIL1β) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de IL-1 beta/IL1B (E7-2-hIL1β): sc-32294.
  • m-IgG Fc BP-HRP e m-IgGκ BP-HRP são os reagentes de deteção secundários preferidos para IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) (ver informações para encomenda abaixo).

LINKS RÁPIDOS

VEJA TAMBÉM

O anticorpo IL-1β (E7-2-hIL1β) é um anticorpo monoclonal de cadeia leve kappa IgG2b de camundongo que detecta IL-1β humana por western blotting (WB), imunoprecipitação (IP), imunofluorescência (IF) e imuno-histoquímica com secções incluídas em parafina (IHCP). O anticorpo monoclonal IL-1β (E7-2-hIL1β) está disponível em formas não conjugadas e em várias formas conjugadas, incluindo agarose, peroxidase de rábano (HRP), ficoeritrina (PE), isotiocianato de fluoresceína (FITC) e vários conjugados Alexa Fluor®. A IL-1β é uma citocina pró-inflamatória chave que medeia as respostas imunitárias e a inflamação. A IL-1β é secretada principalmente por macrófagos ativados e ativa os linfócitos T e B, bem como as células natural killer (NK). A IL-1β reside no citoplasma e é libertada após ativação celular, ligando-se ao recetor de IL-1 para iniciar vias de sinalização que produzem outros mediadores inflamatórios. Esta localização citoplasmática permite uma resposta rápida a estímulos inflamatórios, tornando a IL-1β essencial nos mecanismos de defesa do organismo. A desregulação da produção de IL-1β tem sido associada a várias patologias, incluindo artrite reumatoide, doença inflamatória intestinal e aterosclerose, realçando a importância da IL-1β na saúde e na doença.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β)

sc-32294
200 µg/ml
$316.00

Pacote do IL-1 beta/IL1B (E7-2-hIL1β): m-IgG Fc BP-HRP

sc-528454
200 µg Ab; 10 µg BP
$354.00

Pacote do IL-1 beta/IL1B (E7-2-hIL1β): m-IgGκ BP-HRP

sc-520835
200 µg Ab, 40 µg BP
$354.00

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) AC

sc-32294 AC
500 µg/ml, 25% agarose
$416.00

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) HRP

sc-32294 HRP
200 µg/ml
$316.00

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) FITC

sc-32294 FITC
200 µg/ml
$330.00

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) PE

sc-32294 PE
200 µg/ml
$343.00

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) Alexa Fluor® 488

sc-32294 AF488
200 µg/ml
$357.00

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) Alexa Fluor® 546

sc-32294 AF546
200 µg/ml
$357.00

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) Alexa Fluor® 594

sc-32294 AF594
200 µg/ml
$357.00

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) Alexa Fluor® 647

sc-32294 AF647
200 µg/ml
$357.00

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) Alexa Fluor® 680

sc-32294 AF680
200 µg/ml
$357.00

IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) Alexa Fluor® 790

sc-32294 AF790
200 µg/ml
$357.00

I would like to know if this IL-1B antibody ((E7-2-hIL1β): sc-32294) can detect pro-IL-1b and (mature) IL-1B. Thank you

Asked by: Anonymous
Thank you for your question. This antibody can detect both mature and precursor forms of IL1B in western blot.
Answered by: Tech Support Europe
Date published: 2025-08-04

这个抗体 “IL-1β 抗体 (E7-2-hIL1β): sc-32294”能用于检测猪的IL-1β吗?

Asked by: yingyingying
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

can I use this antibody(sc-32294) in mouse brain tissue for western blot?

Asked by: wcl6231
Thank you for your question. This antibody has not yet been tested in mouse and so that is not a recommended species at this time. It is human reactive only. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2020-01-15

Can IL-1β (E7-2-hILβ): sc-32294 monoclonal antibody be used in WB with zebrafish tissue ? Will there be non-specific staining?

Asked by: havana
Thank you for your question. This antibody has not been tested for use on zebrafish samples, so it is not recommended or warrantied for this use. However we do have a publication with this antibody in fish samples (PMID: 30589112). It is fine for WB applications.
Answered by: Tech Service
Date published: 2019-10-28

Can this il- 1b (sc 32294) detects mature form also..please clear

Asked by: Rahul89
Thank you for your question. Yes, this antibody, IL-1β Antibody (E7-2-hIL1β): sc-32294, is recommended for detection of precursor and mature IL-1beta. This has been confirmed through testing against the purified mature IL-1beta protein. In the cell lysates tested, we have also only observed the precursor form. We recommend you consult some of the product citations using this antibody to see if there is any recommendation for inducing expression of the mature form of the protein.
Answered by: Technical Support
Date published: 2019-07-12

Can IL-1β (E7-2-hILβ): sc-32294 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IL-1β (E7-2-hILβ): sc-32294 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from hl-60Because I used rabbit antibody had a good result, so I brought this again, but it didn't work in hl-60 cells.
Date published: 2020-09-19
Rated 1 out of 5 by from inconsistent for western blottingReceived a free sample of this antibody to test on AC16 cell lysates. Very high lot to lot variability. One lot showed only the mature band while the other showed only the precursor band for the same cell type. Antibody dilution used was 1:200.
Date published: 2018-04-27
Rated 2 out of 5 by from not work well for IF staining for rat brain tissueformaldehyde fixed rat brain tissue, get signal,but not good
Date published: 2017-10-24
Rated 5 out of 5 by from IHC stainingThe sample arrived soon and it worked well on the dilution 1:200 in IHC-P
Date published: 2017-10-20
Rated 4 out of 5 by from 此抗体功能很不错已经购买好几次这个抗体了,一如既往的好用。但是也有点缺点,就是有时候此蛋白的切割产物显示不出来,还是以前兔抗的更好用些。
Date published: 2017-09-21
Rated 5 out of 5 by from IL-1β使用评价大概是在八月份申请的试用装,进行WB试验还可以,效果挺不错的,符合试验预期的结果,希望贵公司越做越好
Date published: 2017-09-20
Rated 5 out of 5 by from IHCThe expression of IL-1 beta protein in the brain of rats with cerebral ischemia was analyzed by IHC,This antibody was very useful and we got expected staining which the signal is lower in treated group.1:800 dilution used.
Date published: 2017-09-16
Rated 5 out of 5 by from Very usefulVery useful antibody and it worked great.. Very little background and it is easily got the target band.I suggest use dilution 1:500 to detect this protein.
Date published: 2017-09-02
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IL-1 beta/IL1B Anticorpo (E7-2-hIL1β) is rated 4.1 out of 5 by 34.
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