Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ig J Chain Double Nickase Plasmid (h): sc-403642-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ig J Chain Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Ig J Chain Double Nickase Plasmid (h) and Ig J Chain Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting JCHAIN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ig J chain Antibody (F-12): sc-133177
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ig J Chain Double Nickase Plasmid (h) | sc-403642-NIC | 20 µg | $410.00 | |||
Ig J Chain Double Nickase Plasmid (h2) | sc-403642-NIC-2 | 20 µg | $410.00 |
JCHAIN encodes the immunoglobulin J chain, a small secreted polypeptide that enables polymerization of IgA and IgM by linking Fc regions and promoting assembly of dimeric IgA and pentameric IgM. This polymerization supports mucosal immunity and efficient transport of polymeric immunoglobulins across epithelia via the polymeric immunoglobulin receptor, integrating with B cell differentiation and plasma cell secretory programs. JCHAIN expression is tightly coupled to antibody-secreting cell states and class-switch outcomes, providing a functional readout of humoral immune maturation. Dysregulated JCHAIN levels have been associated with altered mucosal defense, immune dyscrasias, and B cell–derived malignancies where immunoglobulin production and secretion pathways are perturbed.
Ig J Chain Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the JCHAIN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within JCHAIN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt JCHAIN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of JCHAIN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.