H2-I-A/I-E Antibody (M5/114): sc-23940
- H2-I-A/I-E Antibody (M5/114) is a rat monoclonal IgG2b H2-I-A/I-E antibody provided at 200 µg/ml
- raised against activated C57BL/6 mouse spleen cells
- H2-I-A/I-E Antibody (M5/114) is recommended for detection of I-Ab, I-Ad, I-Aq, I-Ed and I-Ek MHC class II alloantigens of mouse origin by IF and FCM; non cross-reactive with I-Af, I-Ak or I-As; detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes, but not mice carrying the H-2s or H-2f
- Anti-H2-I-A/I-E Antibody (M5/114) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for H2-I-A/I-E Antibody (M5/114). This work is in progress.
H2-I-A/I-E Antibody (M5/114) is a rat monoclonal IgG2b antibody that detects H2-I-A/I-E protein of mouse origin by immunofluorescence (IF) and flow cytometry (FCM). Anti-H2-I-A/I-E antibody (M5/114) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. H2-I-A/I-E protein plays a crucial role in the immune system as a major histocompatibility complex (MHC) class II molecule, which is essential for presenting foreign peptides to cytotoxic T lymphocytes, thereby initiating an immune response. The structural characteristics of MHC class II molecules, including their ability to bind and present a diverse array of peptides, are vital for the activation of T lymphocytes, which are key players in adaptive immunity. M5/114 (M5/114.15.2) monoclonal antibody specifically reacts with mouse MHC class II glycoproteins encoded by the I-A and I-E subregions, including I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, while showing no reaction with I-Af, I-Ak, or I-As. Anti-H2-I-A/I-E antibody (M5/114) recognizes a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice with the H-2b, H-2d, H-2q, H-2p, H-2r, and H-2u haplotypes, but not from those with the H-2s or H-2f haplotypes. Additionally, H2-I-A/I-E monoclonal antibody (M5/114) has been reported to inhibit I-A-restricted T cell responses in mice with the H-2b, H-2d, H-2q, and H-2u haplotypes, highlighting its potential utility in immunological research and therapeutic applications.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
H2-I-A/I-E Antibody (M5/114) References:
- Polymorphism and evolution of HLA class I and II genes and molecules. | Little, AM. and Parham, P. 1999. Rev Immunogenet. 1: 105-23. PMID: 11256568
- Accessory proteins that control the assembly of MHC molecules with peptides. | Van Kaer, L. 2001. Immunol Res. 23: 205-14. PMID: 11444385
- The major histocompatibility complex of the rat (Rattus norvegicus). | Günther, E. and Walter, L. 2001. Immunogenetics. 53: 520-42. PMID: 11685465
- Molecular genetics of the HLA complex. | Fischer, GF. and Mayr, WR. 2001. Wien Klin Wochenschr. 113: 814-24. PMID: 11732117
- A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. | Bhattacharya, A., et al. 1981. J Immunol. 127: 2488-95. PMID: 6170707
- A single monoclonal anti-Ia antibody inhibits antigen-specific T cell proliferation controlled by distinct Ir genes mapping in different H-2 I subregions. | Germain, RN., et al. 1982. J Immunol. 128: 1409-13. PMID: 6173436
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
H2-I-A/I-E Antibody (M5/114) | sc-23940 | 200 µg/ml | $322.00 | |||
H2-I-A/I-E Antibody (M5/114) AC | sc-23940 AC | 500 µg/ml, 25% agarose | $424.00 | |||
H2-I-A/I-E Antibody (M5/114) HRP | sc-23940 HRP | 200 µg/ml | $322.00 | |||
H2-I-A/I-E Antibody (M5/114) FITC | sc-23940 FITC | 200 µg/ml | $336.00 | |||
H2-I-A/I-E Antibody (M5/114) PE | sc-23940 PE | 200 µg/ml | $349.00 | |||
H2-I-A/I-E Antibody (M5/114) Alexa Fluor® 488 | sc-23940 AF488 | 200 µg/ml | $364.00 | |||
H2-I-A/I-E Antibody (M5/114) Alexa Fluor® 546 | sc-23940 AF546 | 200 µg/ml | $364.00 | |||
H2-I-A/I-E Antibody (M5/114) Alexa Fluor® 594 | sc-23940 AF594 | 200 µg/ml | $364.00 | |||
H2-I-A/I-E Antibody (M5/114) Alexa Fluor® 647 | sc-23940 AF647 | 200 µg/ml | $364.00 | |||
H2-I-A/I-E Antibody (M5/114) Alexa Fluor® 680 | sc-23940 AF680 | 200 µg/ml | $364.00 | |||
H2-I-A/I-E Antibody (M5/114) Alexa Fluor® 790 | sc-23940 AF790 | 200 µg/ml | $364.00 |