Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GSTM1 Double Nickase Plasmid (h): sc-418201-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GSTM1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GSTM1 Double Nickase Plasmid (h) and GSTM1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GSTM1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GSTM1 Antibody (1H4F2): sc-517262
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GSTM1 Double Nickase Plasmid (h) | sc-418201-NIC | 20 µg | $410.00 | |||
GSTM1 Double Nickase Plasmid (h2) | sc-418201-NIC-2 | 20 µg | $410.00 |
Human GSTM1 encodes glutathione S-transferase mu 1, a phase II detoxification enzyme that catalyzes glutathione conjugation to electrophilic xenobiotics and endogenous reactive metabolites. By limiting oxidative stress and lipid peroxidation–derived adducts, GSTM1 contributes to redox homeostasis and cellular defense pathways that intersect with NRF2-regulated antioxidant responses. Genetic loss or reduced activity of GSTM1 has been associated with inter-individual differences in chemical susceptibility and inflammatory signaling, and is frequently studied in the context of carcinogen metabolism and oxidative damage in airway and liver biology. Altered GSTM1 function is also relevant to research on environmental exposure responses and drug metabolism variability in human cells.
GSTM1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GSTM1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GSTM1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GSTM1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GSTM1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.