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GR/NR3C1/Glucocorticoid Receptor Antibody (G-5) - Western Blotting - Image 391357
Anticorpo GR (G-5) Alexa Fluor® 488: sc-393232 AF488. Análise por western blot fluorescente direta da expressão de GR em lisados de células inteiras de A-431 (A), HeLa (C) e NIH/3T3 (D) e extrato nuclear de A-431 (B). Bloqueado com Reagente de Bloqueio UltraCruz®: sc-516214.
Anticorpo GR (G-5) Alexa Fluor® 488: sc-393232 AF488. Análise por western blot fluorescente direta da expressão de GR em lisados de células inteiras de A-431 (A), HeLa (C) e NIH/3T3 (D) e extrato nuclear de A-431 (B). Bloqueado com Reagente de Bloqueio UltraCruz®: sc-516214.

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5): sc-393232

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Fichas de dados
  • GR/NR3C1/Glucocorticoid Receptor Anticorpo G-5é um anticorpo monoclonal produzido em camundongo IgG2b κ GR/NR3C1/Glucocorticoid Receptor anticorpo, citado em 98 publicações, fornecido em 200 µg/ml
  • Produzido contra aminoácidos 121-420 de GR de human origem
  • GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) é recomendado para a detecção de GR α and GR β de mouse, rat e human origem em WB, IP, IF, IHC(P) e ELISA
  • Anticorpo anti-GR/NR3C1/Glucocorticoid Receptor O anticorpo anti-GR/NR3C1/Glucocorticoid Receptor (G-5) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • disponível como reagente TransCruz para ensaios de Gel Supershift e uso em ChIP (sc-393232 X, 200 µg/0.1 ml)
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de GR/NR3C1/Glucocorticoid Receptor (G-5): sc-393232.
  • m-IgG Fc BP-HRP e m-IgG2b BP- HRP são os reagentes de deteção secundários preferidos para GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) (ver informações para encomenda abaixo).

LINKS RÁPIDOS

Produtos auxiliares
Descricao
Identificacao do gene
Ficha de dados e Protocolos
Informações de investigação

VEJA TAMBÉM

O anticorpo GR (G-5) é um anticorpo GR monoclonal de camundongo IgG2b κ (também designado anticorpo receptor de glicocorticoide ou anticorpo NR3C1) que detecta a proteína GR de origem de camundongo, rato e humana por WB, IP, IF, IHC(P) e ELISA. O anticorpo GR (G-5) está disponível na forma de anticorpo anti-GR não conjugado, bem como em várias formas conjugadas de anticorpo anti-GR, incluindo agarose, HRP, PE, FITC e vários conjugados Alexa Fluor®. O receptor de glicocorticoide (GR) é um fator de transcrição expresso de forma ubíqua que medeia os efeitos dos glicocorticoides. A isoforma mais abundante é o GR α. O GR induz ou reprime a expressão de genes em resposta aos glicocorticoides, mediando processos como apoptose, crescimento e diferenciação celular. Uma classe significativa de genes reprimidos pelo GR é controlada pelo fator de transcrição AP-1. Também foi demonstrado que o GR é o fator limitante na indução da expressão gênica por glicocorticoides. Foi revelado que o GR forma um complexo com o HSP 90, tornando o receptor não ligado ao ligante transcritivamente inativo. Mais importante ainda, os GRs mutantes que não possuem o domínio de sinalização permanecem constitutivamente ativos.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5)

sc-393232
200 µg/ml
$316.00

Pacote do GR/NR3C1/Glucocorticoid Receptor (G-5): m-IgG Fc BP-HRP

sc-526231
200 µg Ab; 10 µg BP
$354.00

Pacote do GR/NR3C1/Glucocorticoid Receptor (G-5): m-IgG2b BP-HRP

sc-549698
200 µg Ab; 10 µg BP
$354.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) X

sc-393232 X
200 µg/0.1 ml
$316.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) AC

sc-393232 AC
500 µg/ml, 25% agarose
$416.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) HRP

sc-393232 HRP
200 µg/ml
$316.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) FITC

sc-393232 FITC
200 µg/ml
$330.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) PE

sc-393232 PE
200 µg/ml
$343.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) Alexa Fluor® 488

sc-393232 AF488
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) Alexa Fluor® 546

sc-393232 AF546
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) Alexa Fluor® 594

sc-393232 AF594
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) Alexa Fluor® 647

sc-393232 AF647
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) Alexa Fluor® 680

sc-393232 AF680
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) Alexa Fluor® 790

sc-393232 AF790
200 µg/ml
$357.00

RRID?

Asked by: lyh15
Thank you for your question. RRID:AB_2687823
Answered by: Ling Chen
Date published: 2024-12-25

I have delta GR in which we have only the c-terminus of GR from 506 aa to the 794 aa so which antibody would be suitable for this deleted version of GR?

Asked by: KIRTIRAVAT
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service by phone or email. Please call (800)-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2024-10-17

is this antibody applicable to the IHC/IP/WB plant model system?

Asked by: KIRTIRAVAT
Thank you for your question. GR/NR3C1/Glucocorticoid Receptor Antibody (G-5): sc-393232 is recommended for detection of GR α and GR β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2024-10-15

Setting: Western Blot 20 mcg protein of tissue lysate (mouse tissue); antibody diluted 1:1000 in 5%BSA. Problem: I have a good band at 95 kDa , but also a clear (unexpected) band around 50kDa. What could this be?

Asked by: Arnot
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Does GR antibody (G-5): sc-393232 work in ChIP assay for rat muscle?

Asked by: jymrobin
Thank you for your question. This antibody has been published previously for ChIP applications (PMID: 30994266 and PMID: 30698747). This antibody is reactive with mouse, rat and human. We do not have any data specifically in rat muscle.
Answered by: Technical Support
Date published: 2019-07-18

How well does the antibody work with rat brain tissue for western blotting, and what would be the optimum concentration?

Asked by: nico315
Thank you for your question. We have not yet tested this antibody in rat brain tissue. We do have data in mouse brain tissue extract. The primary antibody was used at the starting dilution of 1:200, and loading 50ug tissue extract.
Answered by: Technical Support
Date published: 2019-07-13

Is the GR ANTIBODY (G-5) X sc-393232X the same as the GR ANTIBODY (G-5) sc-393232 but just at a higher concentration?

Asked by: MegCon
Thank you for your question. Yes, sc-393232 X is provided at a 10x higher concentration. It is provided as 200µg in 0.1ml while the standard product is provided as 200µg in 1ml.
Answered by: Tech Service
Date published: 2019-01-14

Hi, please let me know what concentration of the  GR Antibody (G-5): sc-393232 would be optimal for  Flow Cytometry.  Thank you.

Asked by: nacharya18
Thank you for your question. We have not yet tested this mouse monoclonal, GR Antibody (G-5): sc-393232, for Flow Cytometry so this application is not recommended at this time. We do offer this antibody directly conjugated to a variety of fluorochromes, including: phycoerythrin (sc-393232 PE), fluorescein (sc-393232 FITC), Alexa Fluor® 488 (sc-393232 AF488) or Alexa Fluor® 647 (sc-393232 AF647), 200 µg/ml, for IF, IHC(P) and FCM. If you purchase one of the direct conjugates, we can extend our product warranty to cover the application of Flow Cytometry. A link to our recommended protocol for FCM is below. We recommend using approximately 1 µg of fluorochrome-conjugated antibody to 100 µl of the prepared cell suspension (equal to 1 million cells) to each tube. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-03
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Rated 5 out of 5 by from Works well in mouse brain at 1:100!We bought this antibody last month. We ran a dilution series in mouse brain on free floating 50-micron sections for the following primary antibody dilution amounts: 1:50, 1:100, and 1:200 (+ a no primary control). There was minimal difference between these concentrations and no staining present in the no primary control so we plan to proceed by using it at 1:100.
Date published: 2021-12-14
Rated 4 out of 5 by from Good for WB of mouse hippocampusWe ordered the sample and proved it for WB of mice hippocampal tissue. Gave a nice specific band for the GR as assessed in WT and Emx1-Nr3c1 KO mice.
Date published: 2021-08-03
Rated 4 out of 5 by from Glucocorticoid receptor antibodyGood staining of mice hypothalamus. Sample: mouse cortex fixed with 4% PFA for 16hr at 4℃, followed by sucrose infiltration and OCT embedding. Staining: floatation staining for 48hr at 4℃ (1:300 or 1:500, 300 was better), followed by 2nd antibody staining. FYI, I have not tried shorter staining such as 1hr at RT or overnight at 4℃.
Date published: 2021-07-28
Rated 3 out of 5 by from Glucocorticoid receptor antibodyThis antibody works better in nuclear then in cytoplasmic fraction of liver (dilution 1:250).
Date published: 2021-02-17
Rated 3 out of 5 by from Glucocorticoid receptor antibodyThis antibody works better in nuclear then in cytoplasmic fraction of liver (dilution 1:250).
Date published: 2021-02-17
Rated 5 out of 5 by from Works excelent in westernblotGR was detected in mesenchymal stem cells and used at a dilution of 1;250 1 H at room temp
Date published: 2020-09-18
Rated 5 out of 5 by from PERFECT CHOICEThis antibody works well. Used 1:500 in tbs 0,1%tween to detect GR in different cell line extract (45gs of total proteins; MCF-7 breast cancer cells and KP2 and SUIT2 pancreatic cancer cells), detect only one specific band at 95 kDa. I’ll buy this antibody.
Date published: 2019-09-27
Rated 5 out of 5 by from Works well!It works well for ChIP on both mouse hepatocytes and mouse livers.
Date published: 2019-04-26
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GR/NR3C1/Glucocorticoid Receptor Anticorpo (G-5) is rated 4.1 out of 5 by 28.
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