GM-CSFRα Antibody (G-12): sc-398971

GM-CSFRα Antibody (G-12) is a mouse monoclonal IgG3 antibody that detects GM-CSFRα in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GM-CSFRα, or Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha, plays a crucial role in hematopoiesis and immune response by mediating the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), which is essential for the proliferation, differentiation, and survival of myeloid cells. GM-CSFRα is primarily located on the surface of hematopoietic cells, including monocytes, macrophages, and neutrophils, making GM-CSFRα vital for the regulation of immune responses and inflammation. Understanding GM-CSFRα localization is important because GM-CSFRα expression can influence the activation and function of immune cells, thereby impacting the body′s ability to respond to infections and diseases. Additionally, GM-CSFRα interaction with its common β subunit is necessary for high-affinity binding and subsequent signaling, which underscores GM-CSFRα importance in maintaining effective immune function. Anti-GM-CSFRα antibody (G-12) is an invaluable tool for researchers studying the mechanisms of immune regulation and the role of GM-CSF in various pathological conditions.

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GM-CSFRα Antibody (G-12) References:

1. A cell type-specific constitutive point mutant of the common beta-subunit of the human granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3, and IL-5 receptors requires the GM-CSF receptor alpha-subunit for activation.  |  Jenkins, BJ., et al. 1999. J Biol Chem. 274: 8669-77. PMID: 10085105
2. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and inflammatory stimuli up-regulate secretion of the soluble GM-CSF receptor in human monocytes: evidence for ectodomain shedding of the cell surface GM-CSF receptor alpha subunit.  |  Prevost, JM., et al. 2002. J Immunol. 169: 5679-88. PMID: 12421947
3. Analysis of the 5' promoters for human IL-3 and GM-CSF receptor alpha genes.  |  Akagawa, E., et al. 2003. Biochem Biophys Res Commun. 300: 600-8. PMID: 12504125
4. The human pseudoautosomal GM-CSF receptor alpha subunit gene is autosomal in mouse.  |  Disteche, CM., et al. 1992. Nat Genet. 1: 333-6. PMID: 1363815
5. Human GM-CSF receptor alpha-chain gene is highly polymorphic but not rearranged in AML.  |  Bardy, PG., et al. 1992. Leukemia. 6: 893-7. PMID: 1387692
6. The Ig-like domain of human GM-CSF receptor alpha plays a critical role in cytokine binding and receptor activation.  |  Mirza, S., et al. 2010. Biochem J. 426: 307-17. PMID: 20078425
7. Analysis of mutations in the GM-CSF receptor alpha coding sequence in patients with acute myeloid leukaemia and haematologically normal individuals by RT-PCR-SSCP.  |  Wagner, HM., et al. 1994. Leukemia. 8: 1527-32. PMID: 7522290
8. Expression of two alternatively spliced forms of the 5' untranslated region of the GM-CSF receptor alpha chain mRNA.  |  Chopra, R., et al. 1996. Exp Hematol. 24: 755-62. PMID: 8635532
9. Analysis of the coding sequence for the GM-CSF receptor alpha and beta chains in patients with juvenile chronic myeloid leukemia (JCML).  |  Freeburn, RW., et al. 1997. Exp Hematol. 25: 306-11. PMID: 9131005
10. Cloning and sequencing of an alternative splicing-derived cDNA variant of the GM-CSF receptor alpha subunit, which encodes a truncated protein.  |  Hu, X. and Zuckerman, KS. 1998. Am J Hematol. 58: 145-7. PMID: 9625584

The preferred GM-CSFRα antibody is GM-CSFRα Antibody (S-50): sc-456.