Date published: 2025-9-8

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GAPDH Anticorpo (0411): sc-47724

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  • GAPDH Anticorpo 0411é um anticorpo monoclonal produzido em camundongo IgG1 κ GAPDH anticorpo, citado em 3.428 publicações, fornecido em 200 µg/ml
  • Produzido contra recombinante GAPDH de human origem
  • GAPDH Anticorpo (0411) é recomendado para a detecção de GAPDH of human origin by WB, IP, IF and IHC(P); não recomendado para a deteção de GAPDH of mouse or rat origin
  • Anticorpo anti-GAPDH O anticorpo anti-GAPDH (0411) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de GAPDH (0411): sc-47724.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP e m-IgGκ BP-HRP são os reagentes de deteção secundários preferidos para GAPDH Anticorpo (0411) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com GAPDH Anticorpo (0411) (ver informações para encomenda abaixo).

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VEJA TAMBÉM

O Anticorpo GAPDH (0411) é um anticorpo monoclonal de cadeia leve IgG1 kappa de camundongo criado contra gliceraldeído-3-fosfato desidrogenase (GAPDH) recombinante de origem humana. A GAPDH é uma enzima essencial na glicólise, catalisando a fosforilação oxidativa reversível do gliceraldeído-3-fosfato, um passo essencial no metabolismo dos hidratos de carbono que gera energia para a célula. Embora se localize principalmente no citoplasma, a GAPDH pode se translocar para o núcleo durante a apoptose, onde participa de funções nucleares como a ativação da transcrição, a replicação do DNA e o reparo do DNA, ressaltando seu papel multifuncional nos processos celulares. A GAPDH interage com várias proteínas importantes, incluindo a proteína precursora β-amiloide (APP), que está implicada na patogénese da doença de Alzheimer; a Huntingtina, a proteína alterada na doença de Huntington, indicando um papel nas doenças neurodegenerativas; e a Siah1, uma ubiquitina ligase E3 envolvida na apoptose, destacando a sua participação nas vias de morte celular. Além disso, a expressão de GAPDH é suprarregulada em cancros do fígado, pulmão e próstata, tornando estes tecidos controlos positivos adequados para o anticorpo monoclonal GAPDH (0411). O anticorpo anti-GAPDH (0411) é reativo com amostras humanas e é ideal para aplicações como western blotting (WB) e imunoprecipitação (IP). Com elevada especificidade e afinidade, o anticorpo monoclonal GAPDH (0411) é uma excelente ferramenta para os investigadores que investigam as vias glicolíticas, as funções nucleares da GAPDH, as suas interações com proteínas relacionadas com doenças e o seu papel no cancro, fazendo assim avançar significativamente a investigação nestas áreas críticas.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

GAPDH Anticorpo (0411)

sc-47724
200 µg/ml
$316.00

Pacote do GAPDH (0411): m-IgG Fc BP-HRP

sc-528511
200 µg Ab; 10 µg BP
$354.00

Pacote do GAPDH (0411): m-IgGκ BP-HRP

sc-520906
200 µg Ab, 40 µg BP
$354.00

Pacote do GAPDH (0411): m-IgG1 BP-HRP

sc-542968
200 µg Ab; 20 µg BP
$354.00

GAPDH Anticorpo (0411) AC

sc-47724 AC
500 µg/ml, 25% agarose
$416.00

GAPDH Anticorpo (0411) HRP

sc-47724 HRP
200 µg/ml
$316.00

GAPDH Anticorpo (0411) FITC

sc-47724 FITC
200 µg/ml
$330.00

GAPDH Anticorpo (0411) PE

sc-47724 PE
200 µg/ml
$343.00

GAPDH Anticorpo (0411) Alexa Fluor® 488

sc-47724 AF488
200 µg/ml
$357.00

GAPDH Anticorpo (0411) Alexa Fluor® 546

sc-47724 AF546
200 µg/ml
$357.00

GAPDH Anticorpo (0411) Alexa Fluor® 594

sc-47724 AF594
200 µg/ml
$357.00

GAPDH Anticorpo (0411) Alexa Fluor® 647

sc-47724 AF647
200 µg/ml
$357.00

GAPDH Anticorpo (0411) Alexa Fluor® 680

sc-47724 AF680
200 µg/ml
$357.00

GAPDH Anticorpo (0411) Alexa Fluor® 790

sc-47724 AF790
200 µg/ml
$357.00

Hello. I have a question. Which do part of Gapdh (amino acid) make your Anti-GAPDH antibody (0411) sc-47724 product?

Asked by: Khurlee
Apple
Answered by: Charlie
Date published: 2025-06-05

we have GAPDH ANTIBODY SC-47724 (LOT a1821). Is it HRP CONJUGATED?

Asked by: ANURADHA
Thank you for your question. Lot A1821 is for the unconjugated form.
Answered by: BlakeJ
Date published: 2024-09-26

What is the amino acid sequence that this GAPDH(0411) recognizes?

Asked by: Vishal Prajapat
Thank you for your question. No epitope mapping has been performed for this antibody as of yet. Its exact binding site is not known.
Answered by: Tech Support Europe
Date published: 2024-01-09

I have not can detect GAPDH-488 (sc-47724 AF488) Can you help me with the protocol?

Asked by: JessicaM
Thank you for your question. Please reach out to our technical service team directly for assistance with your experiment.
Answered by: Tech Support Europe
Date published: 2022-11-30

Hello, This is my first time doing WB, I was wondering if I could get a sample of GAPDH Antibody (0411): sc-47724 to test? I would love to purchase after testing it on our sample? Thanks

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a FREE 10 µg sample of this product.
Answered by: Technical Service 3
Date published: 2024-12-12

Has anyone had successful results using this antibody for the detection of mouse GAPDH?

Asked by: DF60
Thank you for your question. This antibody is not recommended for detection of GAPDH of mouse or rat origin. We have several options of alternative GAPDH antibodies for mouse and rat. We recommend, sc-365062: GAPDH (G-9), which has published Western blot data in mouse and rat on our website. Plus, 40 citations each for mouse and rat.
Answered by: Technical Support
Date published: 2024-09-26

I have a GAPDH antibody SC-20357 (lot# G1807). how old is it? what is the expiration time of your antibodies?

Asked by: Josee
Thank you for your question. This lot was released within the last few months. We do not characterize an expiration date for our antibodies. They can last for several years when stored properly at 4 degrees C (and never frozen). We do warranty our antibodies for one year from the date of purchase.
Answered by: Technical Support
Date published: 2021-08-22

about GAPDH antibody SC-47724, what volume per tube? I know it is 200 ug/ml but I can't find the amount send. thanks

Asked by: Josee
Thank you for your question. We provide this antibody as 200 µg in 1 mL volume.
Answered by: Technical Support
Date published: 2025-01-08
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Rated 5 out of 5 by from Great GAPDH AntibodyGreat GAPDH Antibody (GAPDH (0411), mouse monoclonal Ab. sc-47724; LOT# J0824). The shipping to Thailand was really fast. The quality is good at a reasonable price.
Date published: 2025-05-06
Rated 5 out of 5 by from Reliable loading controlWe regularly use GAPDH as a loading control, and it is always reliable.
Date published: 2024-08-07
Rated 5 out of 5 by from GAPDH works well for WBGAPDH works well for WB in d20 (hESC beta-like) clusters at 1/5000.
Date published: 2024-05-27
Rated 5 out of 5 by from Excelent antibodyThe antibody works wells in western blot. When HT29 cell lysates were used a clear band was detected with the anti-Gapdh at the dilution 1:500.
Date published: 2024-02-20
Rated 1 out of 5 by from Used to work but no longer doesUsed this happily for several years but latest batch is not properly conjugated to HRP. No band unless I use a secondary antibody. Lab manager tried to resolve with company but they are not being forthcoming.
Date published: 2024-02-13
Rated 1 out of 5 by from No hubo reconocimiento de la proteinaLo utilicé en una línea celular de mosquito (C6/36) y no hubo reconocimiento de la proteína
Date published: 2023-10-09
Rated 5 out of 5 by from GAPDH ANTIBOYThe antibody is good on western blot. The band is clean and clear on western blot.
Date published: 2023-08-14
Rated 5 out of 5 by from Works very wellWe bought this one 1 month ago. This antibody works very well in Western blot.
Date published: 2023-06-21
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GAPDH Anticorpo (0411) is rated 4.7 out of 5 by 256.
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