Date published: 2025-9-10

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GAL4 [DBD] Anticorpo (RK5C1): sc-510

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Fichas de dados
  • GAL4 [DBD] Anticorpo RK5C1é um anticorpo monoclonal produzido em camundongo IgG2a κ GAL4 [DBD] anticorpo, citado em 466 publicações, fornecido em 200 µg/ml
  • ativado contra os aminoácidos 94-147 de GAL4
  • GAL4 [DBD] Anticorpo (RK5C1) é recomendado para a detecção de GAL4 DNA binding domain por WB e IP
  • Anticorpo anti-GAL4 [DBD] O anticorpo anti-GAL4 [DBD] (RK5C1) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • available conjugated to biotin for WB, IHC(P) and ELISA
  • disponível como reagente TransCruz para ensaios de Gel Supershift e uso em ChIP (sc-510 X, 200 µg/0.1 ml)
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de GAL4 [DBD] (RK5C1): sc-510.
  • O m-IgG Fc BP-HRP é o reagente de deteção secundário preferido para GAL4 [DBD] Antibody (RK5C1) para aplicações WB. Este reagente é agora oferecido num pacote com GAL4 [DBD] Antibody (RK5C1)(ver informações de encomenda abaixo).

LINKS RÁPIDOS

VEJA TAMBÉM

O anticorpo GAL4 [DBD] (RK5C1) é um anticorpo monoclonal de cadeia leve IgG2a kappa de ratinho que detecta GAL4 por western blotting (WB) e imunoprecipitação (IP). O anticorpo anti-GAL4 (RK5C1) está disponível em formas não conjugadas e em várias formas conjugadas, incluindo agarose, peroxidase de rábano (HRP), ficoeritrina (PE), isotiocianato de fluoresceína (FITC) e vários conjugados Alexa Fluor®. GAL4, derivado de Saccharomyces cerevisiae, actua como um ativador transcricional fundamental, desempenhando um papel crucial na regulação da expressão genética. Os resíduos N-terminais de 147 aminoácidos são essenciais para uma ligação específica e robusta ao ADN, embora estes resíduos não facilitem uma ativação transcricional eficiente de forma independente. Esta caraterística fez do GAL4 uma ferramenta valiosa em biologia molecular, particularmente para a criação de proteínas de fusão que combinam o GAL4 com domínios de ativação de vários reguladores da transcrição. Estas fusões podem ativar eficazmente a transcrição numa série de organismos, incluindo leveduras, plantas, insectos e células de mamíferos. Além disso, o GAL4 tem sido fundamental para o desenvolvimento de um ensaio "two-hybrid", que permite aos investigadores explorar interações proteína-proteína específicas, melhorando assim a nossa compreensão dos processos celulares e dos mecanismos reguladores.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Referencias do GAL4 [DBD] Anticorpo (RK5C1):

  1. Vectores de fusão GAL4 para expressão em células de levedura ou mamíferos.  |  Sadowski, I., et al. 1992. Gene. 118: 137-41. PMID: 1511877
  2. O sistema de dois híbridos: um método para identificar e clonar genes para proteínas que interagem com uma proteína de interesse.  |  Chien, CT., et al. 1991. Proc Natl Acad Sci U S A. 88: 9578-82. PMID: 1946372
  3. A fosforilação dependente da feromona da proteína STE12 da levedura está correlacionada com a ativação transcricional.  |  Song, D., et al. 1991. Genes Dev. 5: 741-50. PMID: 2026326
  4. Activadores e alvos.  |  Ptashne, M. and Gann, AA. 1990. Nature. 346: 329-31. PMID: 2142753
  5. Um novo sistema genético para detetar interacções proteína-proteína.  |  Fields, S. and Song, O. 1989. Nature. 340: 245-6. PMID: 2547163
  6. Um modelo de mecanismo regulador de genes fúngicos: os genes GAL de Saccharomyces cerevisiae.  |  Johnston, M. 1987. Microbiol Rev. 51: 458-76. PMID: 2830478
  7. A análise de deleção do GAL4 define dois segmentos de ativação transcricional.  |  Ma, J. and Ptashne, M. 1987. Cell. 48: 847-53. PMID: 3028647

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

GAL4 [DBD] Anticorpo (RK5C1)

sc-510
200 µg/ml
$316.00

Pacote do GAL4 [DBD] (RK5C1): m-IgG Fc BP-HRP

sc-525422
200 µg Ab; 10 µg BP
$354.00

GAL4 [DBD] Anticorpo (RK5C1) X

sc-510 X
200 µg/0.1 ml
$316.00

GAL4 [DBD] Anticorpo (RK5C1) AC

sc-510 AC
500 µg/ml, 25% agarose
$416.00

GAL4 [DBD] Anticorpo (RK5C1) HRP

sc-510 HRP
200 µg/ml
$316.00

GAL4 [DBD] Anticorpo (RK5C1) FITC

sc-510 FITC
200 µg/ml
$330.00

GAL4 [DBD] Anticorpo (RK5C1) PE

sc-510 PE
200 µg/ml
$343.00

GAL4 [DBD] Anticorpo (RK5C1) Alexa Fluor® 488

sc-510 AF488
200 µg/ml
$357.00

GAL4 [DBD] Anticorpo (RK5C1) Alexa Fluor® 546

sc-510 AF546
200 µg/ml
$357.00

GAL4 [DBD] Anticorpo (RK5C1) Alexa Fluor® 594

sc-510 AF594
200 µg/ml
$357.00

GAL4 [DBD] Anticorpo (RK5C1) Alexa Fluor® 647

sc-510 AF647
200 µg/ml
$357.00

GAL4 [DBD] Anticorpo (RK5C1) Alexa Fluor® 680

sc-510 AF680
200 µg/ml
$357.00

GAL4 [DBD] Anticorpo (RK5C1) Alexa Fluor® 790

sc-510 AF790
200 µg/ml
$357.00

GAL4 [DBD] Anticorpo (RK5C1) B

sc-510 B
200 µg/ml
$320.00

Does this antibody work for flow cytometry?

Asked by: ABLab
Thank you for your question. sc-510 GAL4 [DBD] Antibody (RK5C1) can be used for Flow cytometry,WB, IP,IF and IHC. Here is our flow cytometry protocol : https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-10-04

Hello. I would like to test a free sample of this antibody. Would you mind telling me how to proceed? Yours

Asked by: bfav
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-12-16

Does this antibody work in ICC/IHC?

Asked by: sciencebeetle
Thank you for your question. We have not yet tested this antibody for IF or IHC(P) and so these are not recommended applications at this time. However, we are aware of almost 20 different publications that cite use of the antibody for IF or IHC so we can extend our warranty to cover this application. Please contact Technical Service if you would like a list of the specific product citations, or use the product citation search on our website and you can search by application, species and antibody.
Answered by: Technical Support
Date published: 2018-12-14

Hello, Would it be possible to receive a free sample of this antibody?

Asked by: YuliaLampi
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Dear Sir/Madam, I would like to order a Gal4 antibody for immunohistochemistry in plant protoplast extracts. Would it be possible to receive a free sample of GAL4 (RK5C1): sc-510 to test it before buying it?

Asked by: Dmitri
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Dear Sir/Madam, I would like to order a Gal4 antibody for immunohistochemistry in zebrafish embryos. Would it be possible to receive a free sample of GAL4 (RK5C1): sc-510 to test it before buying it? Many thanks, Florence GIGER, PhD.

Asked by: FloG
Thank you for the question. Yes, we will contact you by email to provide a FREE 10 µg sample of GAL4 (RK5C1): sc-510.
Answered by: Technical Support Europe
Date published: 2017-12-27

I am using this antibody to try to detect yeast two hybrid Gal4DBD-fusion protein expression and I have not been able to detect any even with a high copy vector. What is the WB protocol used for this antibody? Dissolved in BSA or Milk? What concentration?

Asked by: aloha
Thank you for your question. We recommend a starting dilution of 1:200 with this antibody, and we recommend diluting in 5% milk with TBS-T. Here is a link to the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-12-08

Does this antibody work for Drosophila UAS/Gal4 system? Has anyone tried it?

Asked by: sameerbau
Thank you for your question. This monoclonal antibody, GAL4 (RK5C1): sc-510, is recommended for detection of GAL4 DNA binding domain, and is not species-specific. We are aware of several publications specifically using the antibody in a drosophila model. Please refer to the following publications on NCBI's PubMed as a reference, PMID's: 12819212, 10766736 and 12514098.
Answered by: Technical Support
Date published: 2017-06-27
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Rated 5 out of 5 by from Good antibody for immunoprecipitationI used this antibody for immunoprecipitation assay of transfected 293T cells and it worked very well. For 0.5 ml lysates I used 4 ul of antibody.
Date published: 2022-11-06
Rated 4 out of 5 by from good antibodyI use 1:200 in HEK293T transfected with GAL4 plasmid. It works very well.
Date published: 2020-08-25
Rated 5 out of 5 by from Gal4 antibody that works!we tried a free sample of this antibody since our invitrogen one did not seem to work on cell lysates
Date published: 2018-02-16
Rated 5 out of 5 by from Works both on WB and IPI have used this antibody before as agarose conjugate but this was my first time using it for both IP and WB. It works very well without any background. For WB I used it at 1:2000 in TBS O/N and for IP I used 2 ug for 250 ug of nuclear extracts.
Date published: 2017-08-27
Rated 5 out of 5 by from Excellent choiceHighly reliable antibody that is well cited in literature and performs cleanly, especially in Western Blot.
Date published: 2017-01-12
Rated 5 out of 5 by from Able to publish using the antibody in WBAble to publish using the antibody in WB in HEK293 cells and mouse 3T3-L1 cells.
Date published: 2015-09-23
Rated 4 out of 5 by from Strong band in WB and IHC in frican green monkeyStrong band in WB and IHC in frican green monkey, mouse, human COS-7, L cells, P19, HEK 293, 293T. -SCBT Publication Review
Date published: 2015-04-11
Rated 4 out of 5 by from Robust signal in WB in yeast recombinantRobust signal in WB in yeast recombinant GAL4 fusion protein. -SCBT QC
Date published: 2015-03-30
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GAL4 [DBD] Anticorpo (RK5C1) is rated 4.5 out of 5 by 11.
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