Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GABAA Rα5 Double Nickase Plasmid (h): sc-402696-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GABAA Rα5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GABAA Rα5 Double Nickase Plasmid (h) and GABAA Rα5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GABRA5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GABAA Rα5 Antibody (A-5): sc-393921
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GABAA Rα5 Double Nickase Plasmid (h) | sc-402696-NIC | 20 µg | $410.00 | |||
GABAA Rα5 Double Nickase Plasmid (h2) | sc-402696-NIC-2 | 20 µg | $410.00 |
GABRA5 encodes the α5 subunit of the human GABA_A receptor, a ligand-gated chloride channel that mediates inhibitory neurotransmission in the central nervous system. Receptors containing α5 are enriched at extrasynaptic sites and contribute to tonic inhibition, shaping neuronal excitability, synaptic integration, and plasticity through chloride homeostasis and GABAergic signaling. This subunit participates in activity-dependent network regulation and intersects functionally with pathways governing learning and memory circuits. Dysregulation of α5-containing GABA_A receptor signaling has been associated with neuropsychiatric and neurodevelopmental phenotypes, supporting its use in mechanistic studies of inhibitory circuit function.
GABAA Rα5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GABRA5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GABRA5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GABRA5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GABRA5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.