Date published: 2025-9-7

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FOXM1 Anticorpo (G-5): sc-376471

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Fichas de dados
  • FOXM1 Anticorpo G-5é um anticorpo monoclonal produzido em camundongo IgG2b κ FOXM1 anticorpo, citado em 64 publicações, fornecido em 200 µg/ml
  • específico para um epítopo mapeado entre os aminoácidos734-763 no C-terminus de FOXM1 de human origem
  • FOXM1 Anticorpo (G-5) é recomendado para a detecção de all isoforms of FOXM1 de mouse, rat e human origem em WB, IP, IF, IHC(P) e ELISA
  • Anticorpo anti-FOXM1 O anticorpo anti-FOXM1 (G-5) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • disponível como reagente TransCruz para ensaios de Gel Supershift e uso em ChIP (sc-376471 X, 200 µg/0.1 ml)
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de FOXM1 (G-5): sc-376471.
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP e m-IgGκ BP-HRP são os reagentes de deteção secundários preferidos para FOXM1 Anticorpo (G-5) para aplicações WB and IHC(P). Estes reagentes são agora oferecidos em conjuntos com FOXM1 Anticorpo (G-5) (ver informações para encomenda abaixo).

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VEJA TAMBÉM

O anticorpo FOXM1 (G-5) é um anticorpo monoclonal de camundongo IgG2b κ FOXM1 (também designado anticorpo HNF3, anticorpo FKHL16, anticorpo forkhead box M1) que detecta a proteína FOXM1 de origem de camundongo, rato e humana por WB, IP, IF, IHC(P) e ELISA. O anticorpo FOXM1 (G-5) está disponível na forma de anticorpo anti-FOXM1 não conjugado, bem como em várias formas conjugadas de anticorpo anti-FOXM1, incluindo agarose, HRP, PE, FITC e vários conjugados Alexa Fluor®. A família Fox de fatores de transcrição é um grande grupo de proteínas que compartilham um domínio comum de ligação protéica ao DNA denominado hélice com asas ou domínio forkhead. O FOXM1, também conhecido como FKHL16, MPP2 ou Trident, é expresso principalmente em células em proliferação. O gene que codifica o FOXM1 humano é mapeado no cromossomo 12p13. O elemento de transcrição que restringe a expressão do FOXM1 às células em proliferação está localizado 300 pb ascendente ao códon de início. O FOXM1 é mais abundante no timo, testículo, intestino delgado e cólon. As emendas alternativas geram isoformas FOXM1A e FOXM1B que contêm regiões PEST envolvidas na rápida degradação da proteína. Uma diminuição na expressão de FOXM1 está associada a defeitos relacionados à idade na proliferação celular. Por outro lado, um aumento na expressão de FOXM1B nos fígados de camundongos transgênicos mais velhos restaura as taxas de replicação de DNA de hepatócitos para a taxa mais alta presente em fígados jovens. O FOXM1B ativa a transcrição da ciclina B1, ciclina D1 e Cdc25B.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

FOXM1 Anticorpo (G-5)

sc-376471
200 µg/ml
$316.00

Pacote do FOXM1 (G-5): m-IgG Fc BP-HRP

sc-529873
200 µg Ab; 10 µg BP
$354.00

Pacote do FOXM1 (G-5): m-IgGκ BP-HRP

sc-522963
200 µg Ab, 40 µg BP
$354.00

Pacote do FOXM1 (G-5): m-IgG2b BP-HRP

sc-550146
200 µg Ab; 10 µg BP
$354.00

FOXM1 Anticorpo (G-5) X

sc-376471 X
200 µg/0.1 ml
$316.00

FOXM1 Anticorpo (G-5) AC

sc-376471 AC
500 µg/ml, 25% agarose
$416.00

FOXM1 Anticorpo (G-5) HRP

sc-376471 HRP
200 µg/ml
$316.00

FOXM1 Anticorpo (G-5) FITC

sc-376471 FITC
200 µg/ml
$330.00

FOXM1 Anticorpo (G-5) PE

sc-376471 PE
200 µg/ml
$343.00

FOXM1 Anticorpo (G-5) Alexa Fluor® 488

sc-376471 AF488
200 µg/ml
$357.00

FOXM1 Anticorpo (G-5) Alexa Fluor® 546

sc-376471 AF546
200 µg/ml
$357.00

FOXM1 Anticorpo (G-5) Alexa Fluor® 594

sc-376471 AF594
200 µg/ml
$357.00

FOXM1 Anticorpo (G-5) Alexa Fluor® 647

sc-376471 AF647
200 µg/ml
$357.00

FOXM1 Anticorpo (G-5) Alexa Fluor® 680

sc-376471 AF680
200 µg/ml
$357.00

FOXM1 Anticorpo (G-5) Alexa Fluor® 790

sc-376471 AF790
200 µg/ml
$357.00

FOXM1 (G-5) peptídeo neutralizante

sc-376471 P
100 µg/0.5 ml
$68.00

FOXM1 G-5 is mouse monoclonal IgG2b. I would like to know if it can be used with conventional secondary anti-mouse IgG reagents.

Asked by: Gemo
Thank you for your question. Yes, you can use a conventional anti-mouse IgG secondary antibody for detection of this primary antibody. For more specific results, we do recommend using an isotype-specific secondary antibody (anti-mouse IgG2b), or our mouse IgGκ binding protein, e.g., m-IgGκ BP-HRP: sc-516102.
Answered by: Technical Support
Date published: 2020-03-09

Cantidad de anticuerpo FOXM1(G5) X TransCruz sc-376471 recomendado para usar en ChIP.

Asked by: femili12
Gracias por sus preguntas. Recomendamos el uso de 100-500 μg de sobrenadante y 0,1-1 μl de reactivo TransCruz (0,2-2 μg). Para obtener más información, haga clic en este enlace de nuestro protocolo ChIP: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays Thank you for your questions. We recommend using 100–500 µg supernatant and 0.1-1 µl TransCruz reagent (0.2–2 µg). For further information, please click on this link to our ChIP protocol: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays
Answered by: Technical Support
Date published: 2018-12-31

Which type of FOXM1  give good results for IHC on parrafin bock,human tissue breast cancer

Asked by: Madyan
Thank you for your question. We would recommend using our mouse monoclonal antibody, FOXM1 (G-5): sc-376471. We have several pieces of IHC(P) data in human tissue using this antibody on our website.
Answered by: Technical Support
Date published: 2017-08-22

Does the FOXM1 Antibody (G-5): sc-376471 detect isoform “C” of the FOXM1 target?

Asked by: Trav11
Thank you for your question. The FOXM1 Antibody (G-5): sc-376471 will detect all isoforms (A, B, & C) for the FOXM1 protein target. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-16

Does FOXM1 Antibody (G-5): sc-376471 react with Zebrafish samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 13
Date published: 2017-01-13
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Rated 5 out of 5 by from 功能不错,敏感度很高,很好跑半年前试用了一周,感觉效果很棒,再次购买发现没让人失望,效果真的很不错,以后还会再买他家的抗体,都可以试试。
Date published: 2023-10-11
Rated 4 out of 5 by from 经理BACKGROUND The Fox family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXM1, also known as FKHL16, MPP2 or Trident, is primarily expressed in proliferating cells. The gene encoding human FOXM1 maps to chromosome 12p13.33. The transcription element that restricts FOXM1 expression to prolif- erating cells is located 300 bp upstream of the start codon. FOXM1 is most abundant in thymus, testis, small intestine and colon. Alternative splicing generates FOXM1A and FOXM1B isoforms that contain PEST regions involved in rapid protein degradation. A decrease in FOXM1 expression is associated with age-related defects in cellular proliferation. Conversely, an increase in FOXM1B expression in the livers of older transgenic mice restore hepatocyte DNA replication rates to the higher rate present in young livers. FOXM1B activates the transcription of cyclin B1, cyclin D1 and Cdc25B
Date published: 2021-06-23
Rated 5 out of 5 by from the greatest Ab i ever had!it works very well in both KD and OE systems i set up. believe me, commercial ABs of FOXM1 from other companies dont work at all. this showed clear band, at 1:250 dilution with senescent dermal fibroblast cell samples.
Date published: 2020-07-22
Rated 1 out of 5 by from Not specific for FOXM1 - Do not use this antibody!As noticed by other users before, the FOXM1 G-5 antibody detects a protein with the same molecular weight as FOXM1 in Western Blots, but
Date published: 2018-07-18
Rated 4 out of 5 by from Great antibodiesI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 1 out of 5 by from Not sure if observed band is FOXM1Like the previous comments below, our group has also confirmed that this antibody may not be recognizing FOXM1. We observe a strong clear band at around 125-130 kDa with this antibody in our cell lines. However, upon using both siRNA and shRNA approaches with 3 different species, we could not observe a clear knockdown of FOXM1 with this antibody. In contrast, FOXM1 (A-11) sc-271746 showed knockdown of FOXM1 protein. Additionally, while this antibody can recognize overexpressed FOXM1, the exogenous FOXM1 appears as a second at around 110 kDa. The FOXM1 (A-11) antibody only recognizes a single band with much greater intensity. The FOXM1 (A-11) antibody also gave consistent results with CS #D12D5 and an Abcam antibody.
Date published: 2018-05-11
Rated 3 out of 5 by from Gives good band, but uncertain about specificityThe mouse G-5 antibody appears to detect primarily nuclear FOXM1; whereas the SC-500 rabbit antibody detects mainly cytoplasmic (and to some extent nuclear). The expression pattern in western blots, ICC, and IHC have confirmed this (images attached). Possibly due to the G-5 antibody unable to detect FOXM1 in cytoplasm if dimerized? It seems to detect FOXM1 differently in regards to localization; however, the MW appears to be correct. I still haven't quite figured this one out. In any case, I used the antibody at a 1:1000 dilution and secondary at 1:2500. I've noticed most SC antibodies work well at 1:250/500 primary and secondary at 1:2500. It has worked well with overexpression (human FOXM1b isoform) as well as siRNA knockdown in rat cells.
Date published: 2018-03-26
Rated 1 out of 5 by from Not sure the band we detect is FOXM1We have observed similar results than the one showed by elenaenzo with two different siRNA against FOXM1 in HeLa cells (an antibody against FOXM1 from another firm was used as a positive control on the same lysates).
Date published: 2018-03-16
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FOXM1 Anticorpo (G-5) is rated 4.0 out of 5 by 28.
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