Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FMO3 CRISPR/Cas9 KO Plasmid (h): sc-406605
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FMO3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FMO3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FMO3 Antibody (C-1): sc-515042
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FMO3 CRISPR/Cas9 KO Plasmid (h) | sc-406605 | 20 µg | $397.00 |
Overview
FMO3 encodes flavin-containing monooxygenase 3, a microsomal NADPH-dependent monooxygenase highly active in human liver that catalyzes N-oxygenation and S-oxygenation of diverse xenobiotics and endogenous amines. By converting trimethylamine (TMA) to trimethylamine N-oxide (TMAO), FMO3 links dietary nutrient metabolism to hepatic redox biochemistry and systemic metabolite profiles. FMO3 activity contributes to detoxification capacity and influences metabolite-mediated signaling through interactions with broader phase I/phase II biotransformation networks. Genetic or functional perturbation of FMO3 is associated with altered amine oxidation, including trimethylaminuria, and is frequently studied in the context of interindividual variation in drug metabolism and metabolic trait modulation.
FMO3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FMO3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FMO3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FMO3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FMO3 protein expression.
This CRISPR knockout system enables efficient generation of FMO3-deficient cell models for investigation of FMO3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FMO3 exon(s) critical for FMO3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FMO3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FMO3 CRISPR/Cas9 KO Plasmid (h) and FMO3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FMO3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FMO3 HDR Plasmid (h) and FMO3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FMO3 homology arms to support homology-directed repair at defined FMO3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.