eIF5A Double Nickase Plasmid (h): sc-401820-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF5A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF5A Double Nickase Plasmid (h) and eIF5A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EIF5A. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF5A Double Nickase Plasmid (h) | sc-401820-NIC | 20 µg | $410.00 | |||
eIF5A Double Nickase Plasmid (h2) | sc-401820-NIC-2 | 20 µg | $410.00 |
EIF5A encodes eukaryotic translation initiation factor 5A (eIF5A), a highly conserved RNA-binding protein that becomes activated by hypusination and supports productive translation elongation, particularly across polyproline-rich motifs. eIF5A influences mRNA turnover and ribosome dynamics, linking it to proteostasis, cell-cycle progression, and stress-adaptive programs. Through these roles, EIF5A participates in pathways that shape proliferation, apoptosis, and inflammatory signaling outputs. Dysregulation of eIF5A activity or hypusination circuitry has been associated with altered growth control and translational remodeling observed across cancer and other proliferative or stress-related disease contexts.
eIF5A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EIF5A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EIF5A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EIF5A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EIF5A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.