eIF5 Double Nickase Plasmid (h): sc-402133-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF5 Double Nickase Plasmid (h) and eIF5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EIF5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF5 Antibody (E-10): sc-28309
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF5 Double Nickase Plasmid (h) | sc-402133-NIC | 20 µg | $410.00 | |||
eIF5 Double Nickase Plasmid (h2) | sc-402133-NIC-2 | 20 µg | $410.00 |
EIF5 encodes eukaryotic translation initiation factor 5 (eIF5), a core regulator of translation initiation that functions as a GTPase-activating protein for eIF2 to promote start-codon recognition and productive 80S ribosome assembly. By modulating eIF2–GTP hydrolysis and scanning fidelity, eIF5 helps coordinate global protein synthesis with cellular stress responses and growth control, intersecting with pathways such as eIF2 signaling and the integrated stress response. Altered translational control involving eIF5 has been linked to dysregulated proliferation and proteostasis, making EIF5 a useful node for studying how initiation-factor dynamics influence oncogenic and stress-adaptive programs. In human cells, EIF5 perturbation can reveal dependencies in translational reprogramming, mRNA-specific initiation, and stress granule biology.
eIF5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EIF5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EIF5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EIF5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EIF5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.