Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF4AIII Double Nickase Plasmid (m): sc-431393-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF4AIII Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF4AIII Double Nickase Plasmid (m) and eIF4AIII Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Eif4a3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF4AIII Antibody (B-2): sc-365549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4AIII Double Nickase Plasmid (m) | sc-431393-NIC | 20 µg | $410.00 | |||
eIF4AIII Double Nickase Plasmid (m2) | sc-431393-NIC-2 | 20 µg | $410.00 |
Eif4a3 encodes eIF4AIII, a DEAD-box RNA helicase that serves as a core component of the exon junction complex, coupling pre-mRNA splicing to downstream mRNA export, localization, surveillance, and translation. eIF4AIII participates in nonsense-mediated mRNA decay by stabilizing exon junction complex deposition on spliced transcripts and helping define premature termination codons. Through these RNA-processing roles, Eif4a3 influences transcriptome integrity and proteostasis programs that shape cell-cycle control, differentiation, and stress-responsive gene expression. Dysregulation of exon junction complex and NMD-associated factors is linked to developmental and neurobiological phenotypes and is frequently leveraged to study mechanisms of aberrant splicing and RNA quality-control in disease-relevant models.
eIF4AIII Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Eif4a3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Eif4a3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Eif4a3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Eif4a3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.