eIF4AIII Double Nickase Plasmid (h): sc-402660-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF4AIII Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF4AIII Double Nickase Plasmid (h) and eIF4AIII Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EIF4A3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF4AIII Antibody (B-2): sc-365549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4AIII Double Nickase Plasmid (h) | sc-402660-NIC | 20 µg | $410.00 | |||
eIF4AIII Double Nickase Plasmid (h2) | sc-402660-NIC-2 | 20 µg | $410.00 |
EIF4A3 encodes eIF4AIII, a DEAD-box RNA helicase that serves as a core component of the exon junction complex, coupling pre-mRNA splicing to downstream mRNA export, localization, translation, and nonsense-mediated decay. By clamping onto RNA in an ATP-dependent manner, eIF4AIII helps define post-splicing messenger ribonucleoprotein architecture and influences transcript surveillance and proteome fidelity. Altered EIF4A3 function or expression has been linked to dysregulated RNA processing programs relevant to neurodevelopmental phenotypes and cancer-associated changes in gene expression. As a result, EIF4A3 is frequently studied in pathways governing RNA metabolism, genome-wide splicing outcomes, and stress-responsive translational control.
eIF4AIII Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EIF4A3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EIF4A3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EIF4A3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EIF4A3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.