Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF3η CRISPR/Cas9 KO Plasmid (h): sc-400817
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF3η CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the eIF3η genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF3η Antibody (C-5): sc-137214
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF3η CRISPR/Cas9 KO Plasmid (h) | sc-400817 | 20 µg | $397.00 |
Overview
EIF3B encodes a core subunit of the eukaryotic translation initiation factor 3 (eIF3) complex, eIF3η, which coordinates 40S ribosomal subunit recruitment and assembly of the preinitiation complex during cap-dependent translation. Through interactions with additional initiation factors and mRNA, eIF3η supports start-codon selection and controls translation efficiency of transcripts linked to growth, metabolism, and stress adaptation. EIF3B function intersects with pathways regulating proteostasis and cell-cycle progression, and dysregulated translation initiation is frequently associated with oncogenic signaling and altered cellular fitness. As a central node in translational control, EIF3B is commonly studied in contexts of proliferation, stress responses, and transformation-associated changes in protein synthesis programs.
eIF3η CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EIF3B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EIF3B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EIF3B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish eIF3η protein expression.
This CRISPR knockout system enables efficient generation of EIF3B-deficient cell models for investigation of eIF3η signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EIF3B exon(s) critical for eIF3η function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EIF3B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by eIF3η CRISPR/Cas9 KO Plasmid (h) and eIF3η CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EIF3B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by eIF3η HDR Plasmid (h) and eIF3η HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EIF3B homology arms to support homology-directed repair at defined EIF3B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.