Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF2Bδ CRISPR/Cas9 KO Plasmid (m): sc-420143
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF2Bδ CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the eIF2Bδ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF2Bδ Antibody (P-6): sc-9981
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF2Bδ CRISPR/Cas9 KO Plasmid (m) | sc-420143 | 20 µg | $397.00 |
Overview
Eif2b4 encodes the δ subunit of eIF2B, the guanine nucleotide exchange factor that recharges eIF2-GDP to eIF2-GTP and thereby controls translation initiation. By modulating ternary complex availability, eIF2B integrates nutrient and stress cues within the integrated stress response and intersects with eIF2α phosphorylation-dependent regulation of protein synthesis. eIF2B activity influences proteostasis, cellular growth, and adaptive transcriptional programs linked to ER stress and oxidative stress. Dysfunction in eIF2B subunits is associated with hypomyelinating leukodystrophy pathways, making Eif2b4 a relevant node for studying stress-sensitive translation control in neural and glial systems.
eIF2Bδ CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Eif2b4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Eif2b4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Eif2b4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish eIF2Bδ protein expression.
This CRISPR knockout system enables efficient generation of Eif2b4-deficient cell models for investigation of eIF2Bδ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Eif2b4 exon(s) critical for eIF2Bδ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Eif2b4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by eIF2Bδ CRISPR/Cas9 KO Plasmid (m) and eIF2Bδ CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Eif2b4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by eIF2Bδ HDR Plasmid (m) and eIF2Bδ HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Eif2b4 homology arms to support homology-directed repair at defined Eif2b4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.