E2F1 Antibody (KH95): sc-251

E2F-1 Antibody (KH95) is a mouse monoclonal IgG2a kappa light chain antibody that specifically targets E2F-1, a key transcription factor involved in the regulation of cell cycle progression. E2F-1 monoclonal antibody (KH95) is raised against amino acids 342-386 of human E2F-1 and is reactive with E2F-1 from human, mouse, and rat sources, making it suitable for a variety of applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). E2F-1 is a member of the E2F family of transcription factors, which includes E2F-2, E2F-3, E2F-4, E2F-5, E2F-6, and E2F-7, all of which regulate genes critical for the G1 and S phases of the cell cycle. E2F-1 forms functional heterodimers with DP-1, and this complex drives the expression of genes that promote cell cycle progression and DNA replication. E2F-1 is particularly important in regulating the transition from the G1 phase to the S phase, and E2F-1 activity is tightly controlled by interactions with the retinoblastoma protein (Rb). Rb binding inhibits E2F-1 transcriptional activity, preventing the expression of cell cycle genes until appropriate signals trigger Rb inactivation, a process often disrupted in cancer. E2F-1 plays a critical role in both normal cell cycle control and in the dysregulation of cell proliferation seen in various malignancies. E2F-1 monoclonal antibody (KH95) is valuable for studies examining the role of E2F-1 in cell cycle regulation and its involvement in cancer research, as well as in understanding the broader E2F/Rb signaling network.

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E2F1 Antibody (KH95) References:

1. pRb and E2f-1 in mouse development and tumorigenesis.  |  Macleod, K. 1999. Curr Opin Genet Dev. 9: 31-9. PMID: 10072353
2. E2F-1 induced apoptosis.  |  Phillips, AC. and Vousden, KH. 2001. Apoptosis. 6: 173-82. PMID: 11388666
3. A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F.  |  Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
4. E2F-1 induces melanoma cell apoptosis via PUMA up-regulation and Bax translocation.  |  Hao, H., et al. 2007. BMC Cancer. 7: 24. PMID: 17263886
5. The E2F transcription factor is a cellular target for the RB protein.  |  Chellappan, SP., et al. 1991. Cell. 65: 1053-61. PMID: 1828392
6. The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein.  |  Chittenden, T., et al. 1991. Cell. 65: 1073-82. PMID: 1828394
7. E2F-1 overexpression inhibits human gastric cancer MGC-803 cell growth in vivo.  |  Wei, WY., et al. 2015. World J Gastroenterol. 21: 491-501. PMID: 25593464
8. E2F-4, a new member of the E2F transcription factor family, interacts with p107.  |  Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
9. E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo.  |  Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
10. Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members.  |  Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
11. Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation.  |  Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
12. E2F-6, a member of the E2F family that can behave as a transcriptional repressor.  |  Trimarchi, JM., et al. 1998. Proc Natl Acad Sci U S A. 95: 2850-5. PMID: 9501179

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-E2F1 Antibody (KH95) has 769 citations in a variety of scientific publications.

Primary antibodies like Anti-E2F1 Antibody (KH95) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

E2F1 Antibody (KH95) Specifications

Alternate Product Name: E2F1

Product Clone Name: KH95

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 342-386

Epitope Target: E2F-1

Epitope Species: human

Product Reactivity/Detection: E2F-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 769 citations in various scientific publications

Available Conjugates/Forms for E2F1 Antibody (KH95): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent