DGUOK Double Nickase Plasmid (h): sc-407916-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DGUOK Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DGUOK Double Nickase Plasmid (h) and DGUOK Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DGUOK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DGUOK Antibody (H-3): sc-376267
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DGUOK Double Nickase Plasmid (h) | sc-407916-NIC | 20 µg | $410.00 | |||
DGUOK Double Nickase Plasmid (h2) | sc-407916-NIC-2 | 20 µg | $410.00 |
Deoxyguanosine kinase (DGUOK) is a mitochondrial matrix enzyme that phosphorylates deoxyguanosine and deoxyadenosine, providing deoxyribonucleotide precursors required for mitochondrial DNA (mtDNA) replication and repair. By supporting balanced dNTP pools within mitochondria, DGUOK helps maintain mtDNA copy number and integrity, linking nucleotide salvage metabolism to oxidative phosphorylation capacity and mitochondrial biogenesis. Disruption of DGUOK activity is associated with mtDNA depletion and impaired respiratory chain function, making it relevant to studies of mitochondrial maintenance, energy metabolism, and cell stress responses. Human DGUOK is therefore commonly investigated in models of mitochondrial dysfunction and nucleotide homeostasis.
DGUOK Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DGUOK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DGUOK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DGUOK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DGUOK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.