DEC-205 Antibody (PN-15): sc-59158

DEC-205 Antibody (PN-15) is a mouse monoclonal IgG1 antibody that detects DEC-205 in mouse, rat, and human samples through applications such as western blotting (WB), immunofluorescence (IF), and immunohistochemistry. DEC-205 is a crucial multilectin receptor composed of 1,695 residues in its mature form, belonging to the macrophage mannose receptor (MMR) family, which plays a significant role in the immune system by mediating the targeting of membrane receptors to endosomes or lysosomes rich in major histocompatibility complex class II (MHC II) products. DEC-205 is predominantly expressed in mature dendritic cells, featuring a complex structure that includes an extracellular N-terminal cysteine-rich domain, a fibronectin type II domain, and ten C-type carbohydrate recognition domains, along with a single transmembrane region and a small cytoplasmic C-terminal domain. A tyrosine residue at position 1679 within the cytoplasmic domain is particularly noteworthy, as this residue is involved in signaling pathways that can influence immune responses. DEC-205 has the unique ability to either mimic or inhibit interleukin-4 (IL-4) functions, demonstrating a dual role in modulating immune responses by promoting the maturation of epithelial cells or inhibiting the proliferation of T cells. This versatility underscores DEC-205′s importance in immune regulation and potential as a target for therapeutic interventions.

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DEC-205 Antibody (PN-15) References:

1. DEC-205-mediated internalization of HIV-1 results in the establishment of silent infection in renal tubular cells.  |  Hatsukari, I., et al. 2007. J Am Soc Nephrol. 18: 780-7. PMID: 17287423
2. DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp.  |  Pack, M., et al. 2008. Immunology. 123: 438-46. PMID: 17944899
3. Targeting of DEC-205 on human dendritic cells results in efficient MHC class II-restricted antigen presentation.  |  Birkholz, K., et al. 2010. Blood. 116: 2277-85. PMID: 20566893
4. Robust T-cell stimulation by Epstein-Barr virus-transformed B cells after antigen targeting to DEC-205.  |  Leung, CS., et al. 2013. Blood. 121: 1584-94. PMID: 23297134
5. Increased expression of Dec-205, Bcl-10, Tim-3, and Trem-1 mRNA in chronic otitis media with cholesteatoma.  |  Kim, MG., et al. 2014. Acta Otolaryngol. 134: 475-80. PMID: 24702227
6. Targeting DEC-205-DCIR2+ dendritic cells promotes immunological tolerance in proteolipid protein-induced experimental autoimmune encephalomyelitis.  |  Tabansky, I., et al. 2018. Mol Med. 24: 17. PMID: 30134798
7. Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo.  |  Volckmar, J., et al. 2021. J Vis Exp.. PMID: 33616089
8. Elucidating the Motif for CpG Oligonucleotide Binding to the Dendritic Cell Receptor DEC-205 Leads to Improved Adjuvants for Liver-Resident Memory.  |  Li, J., et al. 2021. J Immunol. 207: 1836-1847. PMID: 34479944
9. Development and characterization of DEC-205 receptor targeted Potentilla anserina L polysaccharide PLGA nanoparticles as an antigen delivery system to enhance in vitro and in vivo immune responses in mice.  |  Wu, D., et al. 2023. Int J Biol Macromol. 224: 998-1011. PMID: 36306904
10. cDNA cloning of human DEC-205, a putative antigen-uptake receptor on dendritic cells.  |  Kato, M., et al. 1998. Immunogenetics. 47: 442-50. PMID: 9553150

The preferred DEC-205 antibody is DEC-205 Antibody (F-4): sc-515016.