
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
connexin 40 CRISPR/Cas9 KO Plasmid (h) | sc-401031 | 20 µg | $397.00 |
GJA5 encodes connexin 40 (Cx40), a gap junction protein that assembles into intercellular channels enabling direct passage of ions and small metabolites between neighboring cells. Cx40-mediated coupling supports rapid electrical conduction and coordinated signaling, particularly in the cardiac atria and specialized conduction system, and also contributes to endothelial communication that shapes vascular tone and remodeling. Through regulation of cell–cell connectivity and impulse propagation, altered GJA5 function is linked to arrhythmia-related phenotypes, including atrial conduction abnormalities, and has been studied in the context of congenital cardiac defects and vascular dysregulation.
connexin 40 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GJA5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GJA5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GJA5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish connexin 40 protein expression.
This CRISPR knockout system enables efficient generation of GJA5-deficient cell models for investigation of connexin 40 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
CRISPRs +/- HDRs
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.