Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
connexin 32 Double Nickase Plasmid (h): sc-402378-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- connexin 32 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- connexin 32 Double Nickase Plasmid (h) and connexin 32 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GJB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: connexin 32 Antibody (HAM8): sc-21794
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
connexin 32 Double Nickase Plasmid (h) | sc-402378-NIC | 20 µg | $410.00 | |||
connexin 32 Double Nickase Plasmid (h2) | sc-402378-NIC-2 | 20 µg | $410.00 |
GJB1 encodes connexin 32 (Cx32), a gap junction channel protein that forms intercellular conduits for ions and small metabolites, enabling electrical and metabolic coupling between neighboring cells. Cx32 is prominent in myelinating Schwann cells and other epithelial tissues, where it supports tissue homeostasis by coordinating signaling, redox balance, and responses to injury. Through regulation of gap junctional intercellular communication, connexin 32 influences processes such as membrane excitability, cellular differentiation, and stress signaling pathways. Genetic alterations in GJB1 are strongly associated with inherited peripheral neuropathies, making it a key target for mechanistic studies of myelin maintenance and cell–cell communication defects.
connexin 32 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GJB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GJB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GJB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GJB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.