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CUTL1/CUX1/CDP Antibody (B-10) - Western Blotting - Image 314151
Anticorpo CDP (B-10): sc-514008. Análise por Western blot da expressão de CDP em extractos nucleares de BJAB (A) e K-562 (B).
Anticorpo CDP (B-10): sc-514008. Análise por Western blot da expressão de CDP em extractos nucleares de BJAB (A) e K-562 (B).

CUTL1/CUX1/CDP Anticorpo (B-10): sc-514008

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Fichas de dados
  • CUTL1/CUX1/CDP Anticorpo B-10é um anticorpo monoclonal produzido em camundongo IgG3 κ CUTL1/CUX1/CDP anticorpo, citado em 22 publicações, fornecido em 200 µg/ml
  • específico para um epítopo mapeado entre os aminoácidos1308-1332 no C-terminus de CDP de mouse origem
  • recomendado para a detecção de CDP de mouse, rat e human origem em
  • disponível conjugado à agarose/b> para IP; HRP/b> para WB, IHC(P) e ELISA; ficoeritrina/b>, FITC/b>, Alexa Fluor® 488/b> ou Alexa Flúor® 647/b> para IF, IHC(P) e FCM
  • dispnonível como reagente TransCruz para o uso em ChIP (sc-514008 X, 200 µg/0.1 ml)
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de CUTL1/CUX1/CDP (B-10): sc-514008.
  • m-IgG3 BP-HRP é o reagente de deteção secundária preferido para CUTL1/CUX1/CDP Anticorpo (B-10) para aplicações WB and IHC(P). Este reagente é agora oferecido num pacote com CUTL1/CUX1/CDP Anticorpo (B-10) (ver informações para encomenda abaixo).

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Produtos auxiliares
Descricao
Identificacao do gene
Ficha de dados e Protocolos
Informações de investigação

VEJA TAMBÉM

O CDP Antibody (B-10) é um anticorpo CDP monoclonal de camundongo IgG3 κ (também designado anticorpo CUX1 ou anticorpo CUTL1) que detecta a proteína CDP de origem de camundongo, rato e humana por WB, IP, IF, IHC(P) e ELISA. O anticorpo CDP (B-10) está disponível na forma de anticorpo anti-CDP não conjugado, bem como em várias formas conjugadas de anticorpo anti-CDP, incluindo agarose, HRP, PE, FITC e vários conjugados Alexa Fluor®. A CDP (para a proteína de deslocamento CCAAT) foi identificada como um repressor da transcrição de genes regulados pelo desenvolvimento. Trata-se de uma proteína de homeodomínio que parece competir com as proteínas ativadoras da transcrição pela ligação protéica às regiões promotoras de vários genes. A CDP contém três repetições cortadas que funcionam como domínios de ligação ao DNA. Foi demonstrado que os domínios de repetição de corte têm a capacidade de se ligar ao DNA em conjunto com ou independentemente da ligação do DNA do homeodomínio. Foi demonstrado que a CDP é a subunidade de ligação protéica ao DNA do complexo HiNF-D, que contém ciclina A, Cdc2 e uma proteína relacionada a Rb, além da CDP. A expressão de histonas é necessária para a transição para a fase S do ciclo celular. O complexo HiNF-D regula a transcrição dos genes de histona H4, H3 e H1, permitindo que as células passem da fase G1 para a fase S.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Referencias do CUTL1/CUX1/CDP Anticorpo (B-10):

  1. A proteína de deslocamento CCAAT humana é homóloga à homeoproteína de Drosophila, cut.  |  Neufeld, EJ., et al. 1992. Nat Genet. 1: 50-5. PMID: 1301999
  2. Regulação antagónica do promotor do gene PCNA de Drosophila por DREF e Cut.  |  Seto, H., et al. 2006. Genes Cells. 11: 499-512. PMID: 16629902
  3. A clivagem induzida pela diferenciação de Cutl1/CDP gera uma nova isoforma dominante-negativa que regula a expressão de genes mamários.  |  Maitra, U., et al. 2006. Mol Cell Biol. 26: 7466-78. PMID: 17015474
  4. Os ratinhos transgénicos que expressam a isoforma da proteína de deslocamento p75 CCAAT/Cut homeobox desenvolvem uma leucemia mieloide semelhante a uma doença mieloproliferativa.  |  Cadieux, C., et al. 2006. Cancer Res. 66: 9492-501. PMID: 17018605
  5. As repetições conservadas de cut na proteína homeodomínio cut humana funcionam como domínios de ligação ao ADN.  |  Harada, R., et al. 1994. J Biol Chem. 269: 2062-7. PMID: 7904999
  6. A proteína de homeodomínio do rato Phox2 regula a atividade do promotor Ncam em conjunto com Cux/CDP e é um determinante putativo do fenótipo do neurotransmissor.  |  Valarché, I., et al. 1993. Development. 119: 881-96. PMID: 7910552
  7. A proteína de deslocamento CCAAT compete com múltiplos activadores transcricionais pela ligação a quatro locais no promotor proximal do gp91phox.  |  Luo, W. and Skalnik, DG. 1996. J Biol Chem. 271: 18203-10. PMID: 8663528
  8. CDP/cut é a subunidade de ligação ao ADN do fator de transcrição de genes de histonas HiNF-D: um mecanismo para a regulação de genes no ponto de transição do ciclo celular da fase G1/S independente do fator de transcrição E2F.  |  van Wijnen, AJ., et al. 1996. Proc Natl Acad Sci U S A. 93: 11516-21. PMID: 8876167

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

CUTL1/CUX1/CDP Anticorpo (B-10)

sc-514008
200 µg/ml
$316.00

Pacote do CUTL1/CUX1/CDP (B-10): m-IgG3 BP-HRP

sc-550607
200 µg Ab; 40 µg BP
$354.00

CUTL1/CUX1/CDP Anticorpo (B-10) AC

sc-514008 AC
500 µg/ml, 25% agarose
$416.00

CUTL1/CUX1/CDP Anticorpo (B-10) Alexa Fluor® 488

sc-514008 AF488
200 µg/ml
$357.00

CUTL1/CUX1/CDP Anticorpo (B-10) Alexa Fluor® 647

sc-514008 AF647
200 µg/ml
$357.00

CUTL1/CUX1/CDP Anticorpo (B-10) FITC

sc-514008 FITC
200 µg/ml
$330.00

CUTL1/CUX1/CDP Anticorpo (B-10) HRP

sc-514008 HRP
200 µg/ml
$316.00

CUTL1/CUX1/CDP (B-10) peptídeo neutralizante

sc-514008 P
100 µg/0.5 ml
$68.00

CUTL1/CUX1/CDP Anticorpo (B-10) PE

sc-514008 PE
200 µg/ml
$343.00

CUTL1/CUX1/CDP Anticorpo (B-10) X

sc-514008 X
200 µg/0.1 ml
$316.00

Hi, I used this antibody for my whole mount brain imaging with the technique called iDISCO, and I saw good staining. I saw the signal of Cux1 is mainly in the cytosol instead of the nucleus, which is suggested by the literatures. 

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-11

Hello, I am interested in ordering this antibody for IP. I have some literature citing old polyclonal Ab for IP with great success but the reviews on this monoclonal are worrisome. Also, I am interested in using magnetic beads for labeling. Please advice.

Asked by: sbagla
Thank you for your question. This antibody has been established for IP applications in samples of mouse, rat and human origin in our labs and offer our warranty for this use. If you purchase the antibody and it does not perform as expected in the recommended species and application, please contact our Technical Service Department. If we are unable to help you troubleshoot the antibody, we can provide you with a complimentary replacement antibody, credit or refund. Our warranty is for one year from the date of purchase.
Answered by: Tech Support Europe
Date published: 2019-06-12

Hi there! I've been looking at the wonderful IHC images obtained with the CDP M-222 antibody; however, I'm concerned with the bad reviews of its replacement. I want to use CDP B-10 for PFA fixed free floating mouse brain sections.

Asked by: JuanSA
Thank you for your question. We have not tested this antibody in free floating mouse brain tissue sections. However, we have had excellent results using the antibody in formalin-fixed paraffin-embedded human thyroid and upper stomach tissues. Unlike in some of the customer product reviews, we used the primary antibody at a dilution of 1:50. We do warranty the antibody to work for mouse tissues. If you purchase the antibody and it does not perform as expected in the recommended species and application, please contact our Technical Service Department. If we are unable to help you troubleshoot the antibody, we can provide you with a complimentary replacement antibody, credit or refund. Our warranty is for one year from the date of purchase.
Answered by: Technical Support
Date published: 2018-11-21

What application is the blocking peptide sc-514008 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 7
Date published: 2017-01-22
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Rated 5 out of 5 by from Good antibodyThe antibody arrived quickly. It works well for immunostaining
Date published: 2025-07-07
Rated 1 out of 5 by from No signal at allWe tested this antibody on mouse embryonic brain tissue to stain neocortical upper layers. No signal at all with or without antigen retrieval. It's a shame that M222 antibody was replaced by this one.
Date published: 2020-03-04
Rated 1 out of 5 by from Didn't WorkOur lab has previously used and gotten great results from the SCBT's discontinued polyclonal CDP ab. We purchased the monoclonal since the poly was no longer available, but it did not work. Despite multiple attempts with and without antigen retrieval from two different lot numbers of the antibody, we got no results. SCBT has, however, been extremely helpful in terms of providing a second aliquot of no charge. They responded quickly and we are very happy with their customer service.
Date published: 2019-04-16
Rated 1 out of 5 by from Did not work for mouse brain IHCWe tested this antibody in free floating sections of perfused mouse brain. No specific signal was observed at a 1:100 dilution. Antigen retrieval with citrate buffer pH 6.0 at 85°C for 25 min also failed to produce any specific staining.
Date published: 2018-11-15
Rated 1 out of 5 by from No bands were observed in WBUsed 1:100 dilution for mouse tissue (Liver, brain) as well as AML12 hepatocyte cell line nuclear and cytoplasmic proteins for 1 overnight incubation in 5% milk TBS-T blocking buffer. No bands whatsoever was observed. Actin and Nucleolin band as loading control was very good, so it was not the protein problem. As well, same secondary Ab was used for the Actin that was used with this Ab, so it was not the secondary antibody issue either.
Date published: 2018-03-20
Rated 1 out of 5 by from That's too bad..I got the sample of this and tried. No matter how I tried to stain with this antibody, there's only very blurred signal on the sections
Date published: 2018-02-26
Rated 1 out of 5 by from Did not work for IFTried it for IF on mouse brain slices (1:500) and it did not work
Date published: 2018-01-30
Rated 5 out of 5 by from niceGreat antibody！1：1000 used in the u251 cells by Western Blotting, I got satisfactory results.
Date published: 2017-11-03
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CUTL1/CUX1/CDP Anticorpo (B-10) is rated 2.7 out of 5 by 19.
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