CD9 Antibody (C-4): sc-13118

CD9 Antibody (C-4) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 101-210 of human CD9, reactive with mouse, rat, and human species, and validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). CD9, also known as Tetraspanin-29 or p24, is a type IV transmembrane glycoprotein with four transmembrane domains and is widely expressed on the surface of various cell types, including early B cells, eosinophils, basophils, activated T cells, and platelets. CD9 is integral to cell-cell adhesion, particularly during immune responses and cellular communication. Additionally, CD9 plays a significant role in signal transduction, interacting with low molecular weight GTP-binding proteins to influence intracellular signaling pathways. CD9 is also implicated in the regulation of cell migration, adhesion, and fusion, processes that are critical in immune function, tissue repair, and development. CD9 is associated with a range of pathological conditions and is expressed on non-T acute lymphoblastic leukemia cells and some acute myeloid and chronic lymphoid leukemias, highlighting CD9′s importance as a marker for certain hematological malignancies. As a major component of the platelet surface, CD9 is essential in platelet aggregation and clot formation. Anti-CD9 antibody (C-4) is a reliable tool for studying the function and regulatory mechanisms of this key glycoprotein in health and disease. CD9 (C-4) antibody is available in both non-conjugated and conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® options, providing flexibility for a wide range of experimental applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD9 Antibody (C-4) References:

1. CD9 antigen on acute non-lymphoid leukemia cells: preferential expression by promyelocytic (M3) subtype.  |  Ferrero, D., et al. 1991. Leuk Res. 15: 457-61. PMID: 1861531
2. Evidence that the signal-initiating membrane protein CD9 is associated with small GTP-binding proteins.  |  Seehafer, JG. and Shaw, AR. 1991. Biochem Biophys Res Commun. 179: 401-6. PMID: 1909120
3. cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins.  |  Lanza, F., et al. 1991. J Biol Chem. 266: 10638-45. PMID: 2037603
4. CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin.  |  Masellis-Smith, A. and Shaw, AR. 1994. J Immunol. 152: 2768-77. PMID: 7511626
5. The ins and outs of the transmembrane 4 superfamily.  |  Wright, MD. and Tomlinson, MG. 1994. Immunol Today. 15: 588-94. PMID: 7531445
6. Analysis of CD9, CD32 and p67 signalling: use of degranulated platelets indicates direct involvement of CD9 and p67 in integrin activation.  |  Slupsky, JR., et al. 1997. Br J Haematol. 96: 275-86. PMID: 9029013
7. Upregulation of CD9 expression during TPA treatment of K562 cells.  |  Le Naour, F., et al. 1997. Leukemia. 11: 1290-7. PMID: 9264383
8. Nomenclature and characterization of leukocyte differentiation antigens in ruminants.  |  Naessens, J., et al. 1997. Immunol Today. 18: 365-8. PMID: 9267076

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD9 Antibody (C-4) has 323 citations in a variety of scientific publications.

Primary antibodies like Anti-CD9 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD9 Antibody (C-4) Specifications

Alternate Product Name: CD9

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 101-210

Epitope Target: CD9

Epitope Species: human

Product Reactivity/Detection: CD9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 323 citations in various scientific publications

Available Conjugates/Forms for CD9 Antibody (C-4): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin