CA II Antibody (C-10): sc-166547

CA II Antibody (C-10) is a mouse monoclonal IgG2a antibody that detects carbonic anhydrase II (CA II) in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CA II plays a crucial role in the reversible hydration of carbon dioxide, facilitating essential biological processes such as respiration, acid-base balance, and the formation of various bodily fluids, including saliva and gastric juice. CA II is predominantly located in the cytoplasm, where CA II exhibits the highest turnover rate and broadest tissue distribution among all known human carbonic anhydrase isozymes, making CA II vital for maintaining physiological homeostasis. The human CA2 gene, which encodes CA II, is located on chromosome 8q21, and CA II expression is particularly significant in tissues that require rapid carbon dioxide regulation, such as the kidneys and brain. CA II efficiently catalyzes the conversion of carbon dioxide to bicarbonate and protons, which is essential for processes like bone resorption and calcification, highlighting CA II′s importance in both normal physiology and pathological conditions. Anti-CA II antibody (C-10) is an invaluable tool for researchers studying the diverse roles of carbonic anhydrases in health and disease.

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CA II Antibody (C-10) References:

1. Membrane-associated carbonic anhydrase activity in the kidney of CA II-deficient mice.  |  Ridderstråle, Y., et al. 1992. J Histochem Cytochem. 40: 1665-73. PMID: 1431055
2. Carbonic anhydrase II and alveolar fluid reabsorption during hypercapnia.  |  Chen, J., et al. 2008. Am J Respir Cell Mol Biol. 38: 32-7. PMID: 17690328
3. Carbonic anhydrase II promotes cardiomyocyte hypertrophy.  |  Brown, BF., et al. 2012. Can J Physiol Pharmacol. 90: 1599-610. PMID: 23210439
4. Effects of cryoprotectants on the structure and thermostability of the human carbonic anhydrase II-acetazolamide complex.  |  Aggarwal, M., et al. 2013. Acta Crystallogr D Biol Crystallogr. 69: 860-5. PMID: 23633596
5. Active-site solvent replenishment observed during human carbonic anhydrase II catalysis.  |  Kim, JK., et al. 2018. IUCrJ. 5: 93-102. PMID: 29354275
6. Carbonic anhydrase isoenzymes CA I and CA II in the human eye.  |  Wistrand, PJ., et al. 1986. Invest Ophthalmol Vis Sci. 27: 419-28. PMID: 3081459
7. Metal Ion Binding Induces Local Protein Unfolding and Destabilizes Human Carbonic Anhydrase II.  |  McConnell, KD., et al. 2022. Inorg Chem. 61: 1249-1253. PMID: 34989562
8. Small Molecule Alkoxy Oriented Selectiveness on Human Carbonic Anhydrase II and IX Inhibition.  |  Bozdag, M., et al. 2022. ChemMedChem. 17: e202200148. PMID: 35388618
9. Identification of the inhibition mechanism of carbonic anhydrase II by fructooligosaccharides.  |  Mu, Y., et al. 2024. Front Mol Biosci. 11: 1398603. PMID: 38863966
10. Differentiation-dependent expression of carbonic anhydrase II and III in 3T3 adipocytes.  |  Lynch, CJ., et al. 1993. Am J Physiol. 265: C234-43. PMID: 8338133

The preferred CA II antibody is CA II Antibody (G-2): sc-48351.