Date published: 2025-9-9

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beta Actin Anticorpo (ACTBD11B7): sc-81178

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Fichas de dados
  • beta Actin Anticorpo ACTBD11B7é um anticorpo monoclonal produzido em camundongo IgG1 κ, citado em 1.080 publicações, fornecido em 100 µg/ml
  • activada contra uma proteína recombinante correspondente a uma região próxima da região C-terminus de β-Actin de origem human
  • recomendado para a detecção de β-Actin de mouse, rat e human origem em
  • See beta Actin (C4): sc-47778 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de beta Actin (ACTBD11B7): sc-81178.
  • m-IgG Fc BP-HRP e 1 BP-HRP">m-IgG1 BP-HRP são os reagentes de deteção secundários preferidos para beta Actin Antibody (ACTBD11B7) for WB applications. Estes reagentes são agora oferecidos em conjuntos com beta Actin Antibody (ACTBD11B7)(ver informações de encomenda abaixo).

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VEJA TAMBÉM

O Anticorpo β-Actina (ACTBD11B7) é um anticorpo monoclonal de cadeia leve IgG1 kappa de camundongo criado contra uma proteína recombinante correspondente a uma região próxima do terminal C da β-Actina humana. Especificamente, o anticorpo anti-β-Actina (ACTBD11B7) detecta a β-Actina de origem de camundongo, rato e humana e é adequado para aplicações que incluem western blotting (WB), imunoprecipitação (IP), imunofluorescência (IF) e citometria de fluxo (FCM). A β-Actina é uma proteína citoplasmática altamente conservada que desempenha um papel crucial na manutenção da estrutura, motilidade e integridade das células como componente fundamental do citoesqueleto. A β-Actina está envolvida em vários processos celulares, como a divisão celular, o movimento de vesículas e organelos, a sinalização celular e o estabelecimento e manutenção das junções e da forma das células. A organização dinâmica da β-Actina é regulada por pequenas GTPases como Rho, Rac e Cdc42, que controlam a montagem de fibras de tensão de actina, rufos de membrana e filopódios, respetivamente. Estas interações são essenciais para a reorganização do citoesqueleto e para as respostas celulares a estímulos ambientais. A β-actina interage com numerosas proteínas, incluindo proteínas motoras da miosina e componentes do complexo Arp2/3, facilitando a nucleação e o movimento dos filamentos de actina. A β-actina é expressa de forma ubíqua em todas as células eucarióticas e, juntamente com a γ-actina, constitui as principais isoformas de actina em tecidos não musculares, enquanto a α-actina é encontrada predominantemente em células musculares. Dadas as suas funções essenciais e níveis de expressão constantes, a β-Actina serve como controlo de carga em ensaios de proteínas para garantir uma carga proteica igual nas amostras, tornando o anticorpo anti-β-Actina (ACTBD11B7) uma ferramenta valiosa para os investigadores que estudam a dinâmica do citoesqueleto, a motilidade celular e as vias de sinalização relacionadas.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

beta Actin Anticorpo (ACTBD11B7)

sc-81178
100 µg/ml
$333.00

Pacote do beta Actin (ACTBD11B7): m-IgG Fc BP-HRP

sc-539599
100 µg Ab; 10 µg BP
$354.00

Pacote do beta Actin (ACTBD11B7): m-IgG1 BP-HRP

sc-541587
100 µg Ab; 20 µg BP
$354.00

Does this antibody recognize F-Actin (Filamentous) or G-Actin (Monomeric)?

Asked by: Alejandro
Thank you for your question. β-Actin (ACTBD11B7) antibody does not recognize F-Actin or G- Actin.
Answered by: BlakeJ
Date published: 2025-03-07

Does this product have lyophilized powder form,I got a bottle of this product which contains lyophilized powder. The Lot number is G1311. Thank you.

Asked by: wpihub
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Bom dia,  Você poderia me enviar uma cotação formal em PDF deste produto? Atenciosamente, Amanda Souto Machado

Asked by: AmandaS
Thank you for your question. Please check the distributors details for your country on our web site: https://www.scbt.com/scbt/resources/distributors. They will be happy to provide for you the requested quotation.
Answered by: Technical Support Europe
Date published: 2017-06-14

Could I use sc-516132 m-IgG lambda -HRP as a secondary antibody with sc-81178 (b-Actin (ACTBD11B7)?

Asked by: Cary Stelloh
Thank you for question. β-Actin (ACTBD11B7): sc-81178, is a mouse monoclonal IgG1 with a kappa light chain. Therefore, you could not use the mouse IgG lambda light chain binding protein (sc-516132) as a secondary detection reagent with this primary. Instead, you would want to use our m-IgGκ BP-HRP: sc-516102 (https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp) which specifically recognizes the kappa light chain of mouse IgG.
Answered by: Technical Support
Date published: 2017-02-08

Hi, what is the specific type of this antibody? Is this kappa light chain? I need to decide which scondary antibody is to be used agianst this antibody.

Asked by: baizzang1
Thank you for your question. This is a kappa light chain mouse monoclonal antibody. While most anti-mouse IgG secondary antibodies are appropriate for use with this beta-Actin antibody, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-01-17

What is the recommended secondary reagent for Western blot with this mouse monoclonal primary antibody?

Asked by: Anonymous
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-15

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Anonymous
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. See β-Actin (C4): sc-47778 for β-Actin antibody conjugates, including HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. https://www.scbt.com/scbt/product/beta-actin-antibody-c4
Answered by: Tech Service 6
Date published: 2016-12-15
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Rated 2 out of 5 by from not work wellThis antibodies does not work well. There are multiple bands.
Date published: 2021-01-02
Rated 5 out of 5 by from 功能很出色,结果很赞我之前买的试剂,结果非常棒也很特异,希望santa被更多人认可,被更多杂志接收,造福更多科研工作者,也希望未来有更多机会使用santa的产品
Date published: 2020-03-04
Rated 5 out of 5 by from Great antibodyI just got it few weeks ago, run my blot with it, and it looks great.
Date published: 2019-05-21
Rated 5 out of 5 by from Great antibodyThe antibody works great. It almost gives very specific bands.
Date published: 2019-01-18
Rated 5 out of 5 by from Product qualityI am working in cancer drug resistance. I took cancer drugs from Sigma but my experience was very bad that drugs were not even working. After that, I bought two drugs erlotinib and Gefitinib from Santa Cruz. Both are really working very nicely.
Date published: 2018-11-20
Rated 4 out of 5 by from Good qualityThe main band ans weak smaller one at moderate exposition: tested by Western blot and chemiluminesence detection.
Date published: 2018-06-23
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beta Actin Anticorpo (ACTBD11B7) is rated 4.7 out of 5 by 28.
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