Atm Antibody (G-12): sc-377293

Atm Antibody (G-12) is a mouse monoclonal IgG1 kappa light chain antibody, specifically designed to detect the Ataxia Telangiectasia Mutated (ATM) protein, also known as ATM serine/threonine kinase, of human origin. ATM (G-12) antibody targets the epitope spanning amino acids 2581-2860 of the human ATM protein, making ATM monoclonal antibody (G-12) suitable for a wide range of applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). The ATM protein is a pivotal member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, distinguished by its extensive structure that includes multiple HEAT repeats and a central kinase domain, which are essential for its role in cellular signaling. The intricate architecture of ATM facilitates interaction with key proteins such as p53, BRCA1, and NBS1, enabling coordination of cellular response to DNA double-strand breaks, regulation of cell cycle checkpoints, and promotion of DNA repair processes. These interactions are crucial for maintaining genomic stability and preventing the accumulation of genetic mutations. Understanding the structure and interaction network of ATM is vital, as this knowledge underpins ATM function in DNA damage response pathways and involvement in apoptosis. Mutations in the ATM gene lead to the autosomal recessive disorder ataxia telangiectasia, characterized by neurodegeneration, immune deficiencies, and an increased risk of cancer, highlighting ATM′s essential role in normal cellular function and disease. Anti-ATM antibody (G-12) is an invaluable tool for researchers investigating the molecular mechanisms of ATM, allowing for detailed studies of expression, localization, and interactions within various cellular contexts.

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Atm Antibody (G-12) References:

1. ATM mutations in sporadic lymphoid tumours.  |  Stankovic, T., et al. 2002. Leuk Lymphoma. 43: 1563-71. PMID: 12400598
2. Variations in ATM protein expression during normal lymphoid differentiation and among B-cell-derived neoplasias.  |  Starczynski, J., et al. 2003. Am J Pathol. 163: 423-32. PMID: 12875964
3. ATM signaling and genomic stability in response to DNA damage.  |  Lavin, MF., et al. 2005. Mutat Res. 569: 123-32. PMID: 15603757
4. Identification and characterization of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM.  |  Hickson, I., et al. 2004. Cancer Res. 64: 9152-9. PMID: 15604286
5. Alterations of DNA damage-response genes ATM and ATR in pyothorax-associated lymphoma.  |  Liu, A., et al. 2005. Lab Invest. 85: 436-46. PMID: 15696190
6. Ataxia-telangiectasia-mutated dependent phosphorylation of Artemis in response to DNA damage.  |  Chen, L., et al. 2005. Cancer Sci. 96: 134-41. PMID: 15723659
7. Versatility of the Mec1ATM/ATR signaling network in mediating resistance to replication, genotoxic, and proteotoxic stresses.  |  Corcoles-Saez, I., et al. 2019. Curr Genet. 65: 657-661. PMID: 30610294
8. N6-methyladenosine regulates ATM expression and downstream signaling.  |  Zhang, X., et al. 2021. J Cancer. 12: 7041-7051. PMID: 34729106
9. PIK-related kinases: DNA repair, recombination, and cell cycle checkpoints.  |  Keith, CT. and Schreiber, SL. 1995. Science. 270: 50-1. PMID: 7569949
10. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product.  |  Hartley, KO., et al. 1995. Cell. 82: 849-56. PMID: 7671312
11. Discovery of AT gene sparks biomedical research bonanza.  |  Nowak, R. 1995. Science. 268: 1700-1. PMID: 7792589
12. A single ataxia telangiectasia gene with a product similar to PI-3 kinase.  |  Savitsky, K., et al. 1995. Science. 268: 1749-53. PMID: 7792600

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Atm Antibody (G-12) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-Atm Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Atm Antibody (G-12) Specifications

Alternate Product Name: ATM

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2581-2860

Epitope Target: Atm (ataxia telangiectasia protein)

Epitope Species: human

Product Reactivity/Detection: Atm

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for Atm Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-Atm antibody is p-Atm Antibody (10H11.E12): sc-47739.